Hydrogen peroxide is known to posses a wide range of physiological effects towards functional activity of cells. We have investigated the influence of H2O2 on the activity of glycolys in the native blood cells. Adding of hydrogen peroxide up to final concentration 50 mM led to decrease of activity some glycolytic enzymes. H2O2 inhibited consumption of glucose by blood cells. Despite the fact that H2O2 is well known as prooxidant, we observed decrease of the level of thiobarbituric acid reactive species in the blood cells after their incubation in the presence of hydrogen peroxide for 2 hours. Based on these data we supposed that effects of hydrogene peroxide occur via signal transduction pathways.
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Samokhvalov V.A., Smetanina M.D., Museykina N.Yu., Melnikov G.V., Fedotova O.V., Ignatov V.V. (2003) The influence of low doses of hydrogen peroxide on metabolism of blood cells. Biomeditsinskaya Khimiya, 49(2), 122-127.
Samokhvalov V.A. et al. The influence of low doses of hydrogen peroxide on metabolism of blood cells // Biomeditsinskaya Khimiya. - 2003. - V. 49. -N 2. - P. 122-127.
Samokhvalov V.A. et al., "The influence of low doses of hydrogen peroxide on metabolism of blood cells." Biomeditsinskaya Khimiya 49.2 (2003): 122-127.
Samokhvalov, V. A., Smetanina, M. D., Museykina, N. Yu., Melnikov, G. V., Fedotova, O. V., Ignatov, V. V. (2003). The influence of low doses of hydrogen peroxide on metabolism of blood cells. Biomeditsinskaya Khimiya, 49(2), 122-127.
References
Halliwell В., Gutteridge G.M. (1989) Free Radicals in Biology and Medicine, Clarendon Press, Oxford. Scholar google search
