This is a first report on the presence of creatine kinase, ATP- generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin - thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.
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Kouznetsov D.A., Orlova M.A., Berdieva A.G., Khasigov P.Z. (2003) ATP-generating creatine kinase: a new component discovered newly in viperidae venom. Biomeditsinskaya Khimiya, 49(2), 213-217.
Kouznetsov D.A. et al. ATP-generating creatine kinase: a new component discovered newly in viperidae venom // Biomeditsinskaya Khimiya. - 2003. - V. 49. -N 2. - P. 213-217.
Kouznetsov D.A. et al., "ATP-generating creatine kinase: a new component discovered newly in viperidae venom." Biomeditsinskaya Khimiya 49.2 (2003): 213-217.
Kouznetsov, D. A., Orlova, M. A., Berdieva, A. G., Khasigov, P. Z. (2003). ATP-generating creatine kinase: a new component discovered newly in viperidae venom. Biomeditsinskaya Khimiya, 49(2), 213-217.
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