The effects of inhibitors on complement in vitro and in vivo: elisa analysis of inhibition of a subcomponent C1q and inhibition of complement action on the animal model

Kozlov L.V.1 , Belkin Z.P.1, Bichucher A.M.1, Batalova T.N.1, Dyakov V.L.1

1. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Ministry of Russian Federation
Section: Experimental/Clinical Study
PubMed Id: 14564739
Year: 2003  Volume: 49  Issue: 3  Pages: 284-290
For research of effect of medical product at a system of a complement the methods of analysis of inhibition of a system of a complement in vitro and in vivo are designed. The first of them is ELISA for determination of inhibition of the first stage of complement activation: binding of a subcomponent C1q with immunoglobulin. Second - supression of a lethal action of a mink complement on the mouse. As medical product - inhibitors of a complement the operating known by the inhibiting effect of heparin (known anticoagulant) and suramin (drug for treatment trypanosomiasis) is studied. By the first method was retrieved, that the constant of inhibition of binding suramin with C1q is 411 ± 29 mg/ml (or 0,287 ± 0,020 mmole/l), and the constant for heparin is 36,4 ± 1,7 mg/ml (or 2,28 ± 0,10 mmole/l), i.e. the binding of heparin with C1q in 10 times is better, than those for suramin in weight relation or in 100 times it is better - in molar. The second method is based on capacity of mink serum to kill of mice at the intravenous introducing due to action of a mink complement. Was shown, that the introducing 3 mg of suramin or 0,3 mg of heparin on the mouse cancels killing of mice called by the intravenous introducing 0,08 ml of mink serum, i.e. the efficiency of action of these inhibitors of a complement in experience in vivo also differs in 10 times, and their concentrations in a blood animal approximately correspond to constants of inhibition of C1q binding, obtained method in vitro.
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Kozlov L.V., Belkin Z.P., Bichucher A.M., Batalova T.N., Dyakov V.L. (2003) Biomeditsinskaya khimiya, 49(3), 284-290.
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