Protein C activator from Agkistrodon halys halys venom has been purified by ion-exchange and gel-filtration chromatography. The purified enzyme consists of a single peptide chain with molecular weight of 34 kD. Its pH-optimum was the range of 7,5-8,0. The enzyme was inhibited by DFP, benzamidine, PMSF, EGTA. Protein C activator was as effective as Protac (Pentapharm AG, Switzerland) for determination of protein C level in blood plasma using APTT test and protein C chromogenic substrate.
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Gornitskaya O.V., Platonova T.N. (2003) Purification and characteristic of the protein c activator from Agkistrodon halys halys snake venom. Biomeditsinskaya Khimiya, 49(5), 470-478.
Gornitskaya O.V. et al. Purification and characteristic of the protein c activator from Agkistrodon halys halys snake venom // Biomeditsinskaya Khimiya. - 2003. - V. 49. -N 5. - P. 470-478.
Gornitskaya O.V. et al., "Purification and characteristic of the protein c activator from Agkistrodon halys halys snake venom." Biomeditsinskaya Khimiya 49.5 (2003): 470-478.
Gornitskaya, O. V., Platonova, T. N. (2003). Purification and characteristic of the protein c activator from Agkistrodon halys halys snake venom. Biomeditsinskaya Khimiya, 49(5), 470-478.
References
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