1. Institute of Biomedical Chemistry RAMS 2. Center of Bioengineering, Russian Academy of Sciences 3. RCT&HRB The Ministry of Health of the Russian Federation 4. Peoples' Frendship University
The method of purification Erwinia carotovora recombinant L-asparaginase, expressed in E.coli, including ultrasonic disintegration of biomass, fractionation ammonium sulfate and column chromatography on СМ- or SP-Sepharose has been developed. According to SDS-PAAGE the enzyme preparation was homogeneous, its specific activity and yield consist respectively about 620 IU/mg of protein and 75%. Physical-chemical and structural properties of recombinant Erwinia carotovora L-asparaginase are similar to the enzymes from the wild strains Erwinia carotovora and recombinant L-asparaginase Erwinia chrysanthemi.
Download PDF:
Citation:
Borisova A.A., Eldarov M.A., Zgoon A.A., Alexandrova S.S., Omelyanuk N.M., Sokov B.N., Berezov T.T., Sokolov N.N. (2003) Purification and some properties of recombinant Erwinia carotovora L-asparaginase, expressed in E.coli cells. Biomeditsinskaya Khimiya, 49(5), 502-507.
Borisova A.A. et al. Purification and some properties of recombinant Erwinia carotovora L-asparaginase, expressed in E.coli cells // Biomeditsinskaya Khimiya. - 2003. - V. 49. -N 5. - P. 502-507.
Borisova A.A. et al., "Purification and some properties of recombinant Erwinia carotovora L-asparaginase, expressed in E.coli cells." Biomeditsinskaya Khimiya 49.5 (2003): 502-507.
Borisova, A. A., Eldarov, M. A., Zgoon, A. A., Alexandrova, S. S., Omelyanuk, N. M., Sokov, B. N., Berezov, T. T., Sokolov, N. N. (2003). Purification and some properties of recombinant Erwinia carotovora L-asparaginase, expressed in E.coli cells. Biomeditsinskaya Khimiya, 49(5), 502-507.
Cammack K.A., Marlborough D.I., Miller D.S. (1972) Biochem. J., 126, 361- 379. Scholar google search
Остерман Л.А. (1983) Исследование биологических макромолекул электрофокусированием, иммуноэлектрофорезом и радиоизотопными методами, `Наука`. Scholar google search
