Two methods of measurement of nitrite and nitrate determination in human serum and plasma (a colorimetric method based on Griess reaction and a HPLC method) have been compared HPLC method revealed extremely low level of nitrates blood plasma, which was roughly the same (about 3 mM or less) in healthy donors,and patients with diabetes and hypertension. The level of nitrates in group of healthy donors (29.9 ± 2.8 mM) is lower than in patients with diabetes this level is equal (58.6 ± 6.9 mM), and in patients with hypertension (35.4 ± 2.5 mM).
Comparison of data obtained by colorimetric and HPLC methods revealed close correlation (r=0.77, р < 0,001).
Methodical reuqirements for preparation of samples of serum and plasma of blood for analysis and correct intepretation of results are discussed in view of biochemical properties of nitric oxide molecule
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Kobyliansky A.G., Kuznethsova T.V., Soboleva G.N., Bondarenko O.N., Pogorelova O.A., Titov V.N., Masenko V.P. (2003) Identification of nitric oxide in serum and plasma of blood human. Metod of high pressure liquid chromatography. Biomeditsinskaya Khimiya, 49(6), 597-603.
Kobyliansky A.G. et al. Identification of nitric oxide in serum and plasma of blood human. Metod of high pressure liquid chromatography // Biomeditsinskaya Khimiya. - 2003. - V. 49. -N 6. - P. 597-603.
Kobyliansky A.G. et al., "Identification of nitric oxide in serum and plasma of blood human. Metod of high pressure liquid chromatography." Biomeditsinskaya Khimiya 49.6 (2003): 597-603.
Kobyliansky, A. G., Kuznethsova, T. V., Soboleva, G. N., Bondarenko, O. N., Pogorelova, O. A., Titov, V. N., Masenko, V. P. (2003). Identification of nitric oxide in serum and plasma of blood human. Metod of high pressure liquid chromatography. Biomeditsinskaya Khimiya, 49(6), 597-603.
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