Nitrite (NO2`6) and nitrate (NO36) concentrations are usually measured as a marker of NO metabolism. The aim of this study was to validate and standardize the method for the quantification of these two metabolites (nitrite and nitrate) in serum. The samples (serum) were deproteinized with NaOH, zinc sulfate and reduced with cadmium granules. Analytical recovery of NO36 added to serum samples after reduction with cadmium granules was 96-105% (Griess reaction). Calibration curves for nitrate and nitrite were linear over the concentration range of 2.1 to 200 micromol/L in plasma. The detection limit of the assay was 0.9 micromol/L and the quantitation limit was 2.1 micromol/l . Usual values were determined from healthy subjects: the mean concentration of NO26/NO36`(NOx) was 26.2±1,1 micromol/l (mean±SEM) (n = 39). Within-run precision was 6,8% and between-day precision was 2.1%.
Download PDF:
Citation:
Golikov P.P., Nikolayeva N.Yu. (2004) Method of the measurement of nitrite/nitrate (NOx) in serum. Biomeditsinskaya Khimiya, 50(1), 79-85.
Golikov P.P. et al. Method of the measurement of nitrite/nitrate (NOx) in serum // Biomeditsinskaya Khimiya. - 2004. - V. 50. -N 1. - P. 79-85.
Golikov P.P. et al., "Method of the measurement of nitrite/nitrate (NOx) in serum." Biomeditsinskaya Khimiya 50.1 (2004): 79-85.
Golikov, P. P., Nikolayeva, , N. Yu. (2004). Method of the measurement of nitrite/nitrate (NOx) in serum. Biomeditsinskaya Khimiya, 50(1), 79-85.
References
Johnson M.L., Billiar T.R. (1998) World J. Surg. 22, 187- 196. Scholar google search
Charmandari E., Meadows N., Patel M., Johnston A., Benjamin N. (2001) J. Pediatr. Gastroenterol. Nutr., 32, 423- 427. Scholar google search
Borcherding H., Leikefeld S., Frey C., Diekmann S., Steinrucke P. (2000) Anal. Biochem., 282, 1- 9. Scholar google search
Komori K., Matsumoto Т., Ishida M., Kuma S., Yonemitsu Y., Eguchi D., Sugimachi K. J. (1997) Vase. Surg. 26, 657- 662. Scholar google search
Veda Т., Maekawa Т., Sadamitsu D., Oshita S., Ogino K., Nakamura K. (1995) Electrophoresis., 16, 1002- 1004. Scholar google search
Akiyama K., Kimura A., Suzuki H., Takeyama Y, Gluckman T.L., Terhakopian A., Katagiri Т., Suh K. Y, Roseto J., Bing R.J. (1998) J. Am. Coll. Cardiol., 32, 373- 379. Scholar google search
Hoeldtke R.D., Bryner K.D., McNeill D.R., Hobbs G.R., Riggs J.E., Warehime S.S., Christie I., Ganser G., Van Dyke K. (2002) Diabetes., 51, 2817- 2825. Scholar google search
Brodszki J., Lanne Т., Stale H., Batra S., Marsal K. (2002) BJOG. 109, 546- 552. Scholar google search
Desideri G., Marinucci M.C., Tomassoni G., Masci P.G., Santucci A., Ferri C. (2002) J. Clin. Endocrinol. Metab., 87, 2940- 2945. Scholar google search
Wang Z., Zhu Y. (2001) Zhonghua Fu Chan Ke Za Zhi., 36, 209- 211. Scholar google search
Yu C.M., Fung P.C., Chan G., Lai K. W., Wang Q., Lau C.P. (2001) Am. J. Cardiol., 88, 867- 870. Scholar google search
Gorenflo M., Zheng C, Poge A., BettendorfM., Werle E., Fiehn W., Ulmer H.E. (2001) Clin. Lab., 47,441- 447. Scholar google search
Sanada M., Higashi Y., Nakagawa K., Sasaki S., Kodama I., Sakashita Т., Tsuda M., Ohama K. (2001) Atherosclerosis., 158, 391- 397. Scholar google search
Choi I.С., Fung P.C., Leung A.Y., Lie A.K., Liang R. (2001) Haematologica., 86, 972- 976. Scholar google search
Maeda S., Miyauchi Т., Kakiyama Т., SugawaraJ., Iemitsu М., Irukayama-Tomobe Y., Murakami К., Kumagai Y., Kuno S., Matsuda M. (2001) Life Sci., 69, 1005- 1016. Scholar google search
Hori Т., Matsubara Т., Ishibashi Т., Higuchi K, Ochiai S., Takemoto M., Imai S. (2001) J. Cardiol., 38, 21- 28. Scholar google search
Schulz R., Schmidt D., Blum A., Lopes- Ribeiro X, Lucke C, Mayer K, Olschewski H.,Seeger W., Grimminger F. (2000) Thorax., 55, 1046- 1051. Scholar google search
Strand O.A., Leone A., Gierchky K.E., Kirkeboen K.A. (2000) Crit. Care Med., 28, 2779- 2785. Scholar google search
Cuzzolin L., Lussignoli S., Crivellente E., Adami A., Schena E, Bellavite P., Brocco G., Benoni G. (2000) Int. J. Sports Med., 21, 289- 293. Scholar google search
Lovell S.L., Stevenson H., Young I.S., McDowell G., McEneaney D., Riley M.S., Nicholls D.P. (2000) Eur. J Clin. Invest., 30, 181- 187. Scholar google search
Szaleczky E., Pronai L., Nakazawa H., Tulassay Z. (2000) J. Clin. Gastroenterol., 30, 47- 51. Scholar google search
Cicinelli E., Ignarro L.J., Schonauer L.M., Matteo M.G., Galantino P., Falco N. (1999) Clin. Physiol., 19, 440- 442. Scholar google search
ReimundJ.M., Duclos В., Koehl C, Lehr L, Ezenfis J., Baumann R. (1999) Eur. J. Gastroenterol. Hepatol., 11, 1013- 1018. Scholar google search
Kakoki M., Matsumoto A., Nagata D., Kobayakawa N, Kimura K, Momomura S, Hirata Y. (1999) Clin. Nephrol., 52, 83- 90. Scholar google search
Zunic G., Spasic S., Jelic- Ivanovic Z. (1999) J. Chromatogr. B. Biomed. Sci. Appl., 30, 73- 79. Scholar google search
Albornoz L., Alvarez D., Otaso J.C., Gadano A., Salviu J., Gerona S., Sorroche P., VillamilA., Mastai R. (1999) J. Hepatol., 30, 451- 455. Scholar google search
Wierusz В., Zozulinska D., Kempa M., Skowronski M., Murawska A.(1998) Pol.Arch. Med.Wewn., 100, 139- 144. Scholar google search
Ranta V., Viinikka L., Halmesmaki E., Ylikorkala O.(1999) Obstet.Gynecol., 93, 442- 445. Scholar google search
Minamino T, Kitakaze M, Sanada S, Asanuama H, Kurotobi T, Koretsune Y, Fukunami M, Kuzuya T, Hoki N, Hori M. (1998) Circulation., 98, 1721- 1727. Scholar google search
Avontuur J.A., Boomsma F, van den Meiracker A.H., de Jong F.H., Bruining H.A. (1999) Circulation., 99, 271- 275. Scholar google search
Tsukada Т., Yokoyama K., Arai Т., Takemoto E., Hara S., Yamada A., Kawaguchi Y., Hosoya Т., Igari J. (1998) Biochem. Biophys. Res. Commun., 245, 190- 193. Scholar google search
Catalano M., Carzaniga G., Perilli E., Jun Т., Scandale G., Andreoni S., Carotta M. (1997) Vase. Med., 2, 302- 305. Scholar google search
Minamino Т., Kitakaze M., Sato K., Asanuma H., Funaya H., Koretsune Y., HoriM. (1997) Arterioscler. Thromb. Vase. Biol., 17, 3191- 3195. Scholar google search
Tsikas D., Boger R.H., Bode- Boger S.M., Gutzki F.M., Frolich J.С.J, Chromatogr B. (1994) Biomed. Appl., 18, 185- 191. Scholar google search
Node K., Kitakaze M., Yoshikawa K., Kosaka H., Hori M. (1997) Hypertension., 30, 405- 408. Scholar google search
Spack L., Havens P.L., Griffith O. W. (1997) Crit Care Med., 25, 1071- 1078. Scholar google search
Node K., Kitakaze M, Yoshikawa H, Kosaka H, Hori M. (1997) Am. J. Cardiol., 79, 1538- 1541. Scholar google search
Lopez B.L., Barnett J., Ballas S.K., Christopher T.A., Davis- Moon L., Ma X. (1996) Acad. Emerg. Med., 3, 1098- 1103. Scholar google search
Lin S.H., Chu P., Yu EC, Diang L.K., Lin Y.E. (1996) ASAIO J. 42, 895- 899. Scholar google search
Jaekle R.K., Lutz P.O., Rosenn В., Siddiqi T.A., Myatt L. (1994) Am. J. Obstet. Gynecol., 171, 1115- 1119. Scholar google search
