1. I.M. Sechenov Moscow Medical Academy 2. Russian Institute of Herbal and Aromatic Plants 3. Semenov Institute for Chemical Physics, Russian Academy of Sciences
A simple and efficient technique for fast isolation and purification of a total polyphenol fraction from dry herbal plant extract ("Phytoprost" preparation) has been developed. The method is based on sejuential cascade of 1,0-5,0 kDa ultrafiltration and the following subsejuent column chromatography of resultant oligo/polymeric compounds on Phenyl-Sepharose 4B-CL. This novel procedure allows to obtain a highly purified (not more than 0,5-0,6 % of aromatic and heterocyclic impurities) fraction of plant polyphenols in amounts required for pharmacological/biochemical screening (0,85±0,06 A280 /g dry extract) within 8-9 hours.
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Markarian A.A., Dargaeva T.D., Alyautdin R.N., Bourkov V.L. (2004) The accelerated hydrophobic chromatography of plant extracts: a promising tool for study of biologically active polyphenols. Biomeditsinskaya Khimiya, 50(2), 217-220.
Markarian A.A. et al. The accelerated hydrophobic chromatography of plant extracts: a promising tool for study of biologically active polyphenols // Biomeditsinskaya Khimiya. - 2004. - V. 50. -N 2. - P. 217-220.
Markarian A.A. et al., "The accelerated hydrophobic chromatography of plant extracts: a promising tool for study of biologically active polyphenols." Biomeditsinskaya Khimiya 50.2 (2004): 217-220.
Markarian, A. A., Dargaeva, T. D., Alyautdin, R. N., Bourkov, V. L. (2004). The accelerated hydrophobic chromatography of plant extracts: a promising tool for study of biologically active polyphenols. Biomeditsinskaya Khimiya, 50(2), 217-220.
References
Allbright, K.D., Wilczarek, S.L. (2001) Biophannacy and Plant Polyphenols. Alba Regia Publ.: Szeged - Budapest. Scholar google search
Wang Z.Y., Lee P.K., Thorwaldsen D., Lebreu N.M. (1989) Carcinogenesis, 10, 411- 415. Scholar google search
Namura S., Ohta K., Katoh J., Ueda Y. (2003) J. Pharra. Soc. Japan, 48. 211- 230. Scholar google search
Маркарян А.А., Даргаева Т.Д., Николаев С.М., Николаева Г.Г. (2003) Мат.науч.конф. “Актуальные проблемы инноваций с нетрадиционными природными ресурсами и создания функциональных продуктов”. Москва, с.225. Scholar google search
Leder О.Н., Schmidt К., Sarcar J. (1998) Mass Spec. Res.. 19, 298- 309. Scholar google search
Rattenau V., Thornwall L. (2000) Computat. Chem. Biochem., 6, 91- 114. Scholar google search
Warrenbaum K. (1999) UV - Spectrometry in Biopharmacy and Forensic Studies. Hhrlich Schaumitter: Heidelberg. Scholar google search
Milic M., Kudrja M. (2001) Acta Biol. Med. Slovenica, 10, 811- 820. Scholar google search
