Reduced hepatic mitochondrial β-oxidation and changes in L-carnitine metabolism are important biochemical manifestations of valproate (VA)-induced hepatic toxicity. Lipid peroxidation activation as a possible mechanism implicated in VA - induced damage as well as the possibility of L-carnitine (LC) attenuation of lipid peroxidation activity were studied. The level of malondialdehyde (MDA), lipid peroxide concentration and antioxidant activity (AOA), catalase activity, free S-S groups content in plasma and liver homogenates from male albino rats supplemented with VA (200 mg/kg, 8 days) and VA plus LC (100 mg/kg, 8 days) were measured. There were insignificant differences in MDA formation and catalase activity in the plasma and liver of control and VA - treated groups, however decreases in the plasma AOA activity and S-S groups level were observed in VA-treated rats. The LC administration significantly decreased liver lipid peroxide concentration and increased plasma AOA activity and S-S groups. Our results suggest that lipid peroxidation may be involved as an additional mechanism for VA - induced liver damage in rats. The potential antioxidant activity of LC may be particularly relevant in understanding the pharmacological and biochemical properties of LC in VA - induced pathologic conditions.
Download PDF:
Citation:
Bykov I.L., Maltsev A.N., Gurinovich V.A., Nefyodov L.I. (2004) Biochemical basis of valproic acid toxicity: role of oxidative stress and effects of L-carnitine. Biomeditsinskaya Khimiya, 50(4), 384-389.
Bykov I.L. et al. Biochemical basis of valproic acid toxicity: role of oxidative stress and effects of L-carnitine // Biomeditsinskaya Khimiya. - 2004. - V. 50. -N 4. - P. 384-389.
Bykov I.L. et al., "Biochemical basis of valproic acid toxicity: role of oxidative stress and effects of L-carnitine." Biomeditsinskaya Khimiya 50.4 (2004): 384-389.
Bykov, I. L., Maltsev, A. N., Gurinovich, V. A., Nefyodov, L. I. (2004). Biochemical basis of valproic acid toxicity: role of oxidative stress and effects of L-carnitine. Biomeditsinskaya Khimiya, 50(4), 384-389.
References
Davis R., Peters D.H., Me Tavish D. (1994) Drugs, 42, 332 - 372. Scholar google search
Loscher W., Wahnshaffe K., Honack D. et al. (1992) Epilepsy Res., 13, 187- 198. Scholar google search
Kittle A.E., Rettewmeier A.W., Howard W.N., Baillie T.A. (1987) Science 235, 890- 893. Scholar google search
Kibayashi M., Nagao M., Chiba S. (1999) Pediatr. Int., 41, 52 - 60. Scholar google search
Kassahum К., Hu P., Glillo M.P., et al. (1994) Chem. Biol. Interact., 90, 253- 275. Scholar google search
