Using ThioGlo-1 for the determination of thiols in brain tissue

   
Yakovlev A.A.1, Gulyaeva N.V.2

1. Institute of Higher Nervous Activity and Neurophysiology RAS, Moscow Institute of Physics and Technology
2. Institute of Higher Nervous Activity and Neurophysiology RAS
Section: Experimental/Clinical Study
PubMed Id: 15518186
Year: 2004  Volume: 50  Issue: 4  Pages: 390-397
A technique for the fluorometric determination of protein and non-protein thiols in brain tissue with the maleimide reagent ThioGlo-1is has been described. Comparison with the most popular spectrophotometric Ellman method has been performed. The ThioGlo-1-based method is highly sensitive and may be used for thiol determination both in homogenates and supernatants of brain tissue.
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Yakovlev A.A., Gulyaeva N.V. (2004) Biomeditsinskaya khimiya, 50(4), 390-397.
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