Experimental ischemia of rat myocardium was accompanied by increase of light sum (S) and maximal intensity (Imax) of chemiluminescence, amount of a malonic dialdehyde and conjugated dienes in cytoplasmic fraction. The activity of NADP-dependent malate dehydrogenase (EC 1.1.1.82; NADP-MDH) was 1.6 times higher in rat heart under ischemia. NADP-MDH was purified from normal and ischemia-exposed rat myocardium. Using NADP-MDH purified enzyme preparations the values if Hill coefficient for oxaloacetate (1.83 ± 0.07 and 1.50 ± 0.10) and Km for NADPH (0.058 ± 0.003 and 0.096 ± 0.004 mM) were determined for the enzyme at norm and under ishemia respectively. Effects of Fe2+, Ca2+, Cu2+ ions, H2O2, oxidized and reduced glutathione, adenine nucleotides influence on functioning of NADP-MDH from rat heart at norm and under ischemic conditions have been investigated.
Safonova O.A., Popova T.N., Matasova L.V., Artyukhov V.G. (2005) Free radical processes intensity and regulation of cytoplasmic NADP-dependent malate dehydrogenase in rat cardiomyocytes at norm and under ischemia. Biomeditsinskaya Khimiya, 51(3), 311-320.
Safonova O.A. et al. Free radical processes intensity and regulation of cytoplasmic NADP-dependent malate dehydrogenase in rat cardiomyocytes at norm and under ischemia // Biomeditsinskaya Khimiya. - 2005. - V. 51. -N 3. - P. 311-320.
Safonova O.A. et al., "Free radical processes intensity and regulation of cytoplasmic NADP-dependent malate dehydrogenase in rat cardiomyocytes at norm and under ischemia." Biomeditsinskaya Khimiya 51.3 (2005): 311-320.
Safonova, O. A., Popova, T. N., Matasova, L. V., Artyukhov, , V. G. (2005). Free radical processes intensity and regulation of cytoplasmic NADP-dependent malate dehydrogenase in rat cardiomyocytes at norm and under ischemia. Biomeditsinskaya Khimiya, 51(3), 311-320.
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