The formation of complexes between trypsin (T) and soybean tripsin inhibitor (STI) was analyzed, using a two-channel IAsys* optical biosensor. The temperature dependence of complex formation (with its major parameters – the association rate constants (kon), the dissociation rate constants (koff) and the equilibrium constant (Kp)) was determined. The equilibrium constants obtained well correlate with those obtained by other methods. The association rate constant for the binding of T\STI complex increased with temperature, while the dissociation rate constant practically remained unchanged. The association and dissociation rate constant activation parameters were calculated from their concentration dependence. Based on the temperature dependence the activation energy, enthalpy and entropy of complex formation were calculated. It was shown that the entropy component plays a key role in T\STI interaction
Rachenkova N.I., Ivanov Yu.D., Molnar A.A., Archakov A.I. (2005) Optical biosensor study interaction between trypsin and trypsin inhibitor. Biomeditsinskaya Khimiya, 51(6), 617-625.
Rachenkova N.I. et al. Optical biosensor study interaction between trypsin and trypsin inhibitor // Biomeditsinskaya Khimiya. - 2005. - V. 51. -N 6. - P. 617-625.
Rachenkova N.I. et al., "Optical biosensor study interaction between trypsin and trypsin inhibitor." Biomeditsinskaya Khimiya 51.6 (2005): 617-625.
Rachenkova, N. I., Ivanov, Yu. D., Molnar, A. A., Archakov, , A. I. (2005). Optical biosensor study interaction between trypsin and trypsin inhibitor. Biomeditsinskaya Khimiya, 51(6), 617-625.
Archakov A.I., Ivanov Yu.D., (1999) in: Biophysics of Electron Transfer and Molecular Bioelectronics, Plenium Publication Corporation, pp. 173-194. Scholar google search
Морозов С.Г., Иванов. Ю.Д., Учайкин В.Ф., Каракас Н.В., Вахрушина С.В., Гнеденко О.В., Конев В.А., Ковалев О.Б., Арчаков А.И. (1999) Новости Науки и Техники. Серия Медицина. Аллергия, астма и клиническая иммунология, 9, 187-189. Scholar google search
Ivanov Yu.D., Kanaeva I.P., Kuznetsov V.Yu., Lehnerer M., Schulze J., Hlavica P., and Archakov A.I. (1999) Arch. Biochem. Biophys. 362, 87-93. Scholar google search
Johnsson U., Fagerstam L., Ivarsson B., Johnsson B., Karlsson R., Lundh K., Lofas S., Persson B., Roos H., Ronnberg I., Sjolander S., Stenberg E., Stahlberg R., Urbaniczky C., Ostlin H., and Malmqvist M. (1991) BioTechnniques 11, 620-627. Scholar google search
Cush R., Cronin J.M., Stewart W.J., Maule C.H., Molloy J., Goddard N.J. (1993) Biosensor and Bioelectronics 8, 347-353. Scholar google search
Davies R.J., Edwards P.R., Watts H., Lowe C.R., Buckle P.E., Yeung D., Kinning T.M., and Pollard- Knight D.V. (1994) in Techniques in Protein Chemistry V, Academic Press, Inc, pp. 285-292. Scholar google search
Yeung D., Gill A., Maule C.H., and Davies R.J. (1995) Trends Analyt. Chem. 14, 49-56. Scholar google search
