Electrochemical reduction of cytochrome P450s based on electrodes with immobilized substrates

Shumyantseva V.V.1 , Bulko T.V.1, Kuznetsova G.P.1, Samenkova N.F.1, Bachman T.2, Schulze H.2, Schmid R.D.2, Usanov S.A.3, Archakov A.I.1

1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
2. Institute of Technical Biochemistry Stuttgart University
3. Institute of Bioorganic Chemistry, Belarus National Academy of Science
Section: Experimental/Clinical Study
PubMed Id: 16739920
Year: 2006  Volume: 52  Issue: 1  Pages: 44-51
A new approach for the electrochemical reduction of cytochromes P450 (P450s, CYPs) with electrodes chemically modified with appropriate substrates of P450s ("reverse" electrodes) has been proposed. The method is based on the analysis of cyclic voltammograms, square wave voltammograms, amperograms and determination of such electrochemical characteristics as catalytic current and redox potential. The sensitivity of the proposed method is 0.2-1 nmol P450/electrode. The differences of maximal current and potentials in square wave voltammograms and catalytic current in amperometric measurements are more sensitive and reliable. Planar regime of screen-printed electrodes permits to use 20-60 µl of electrolyte volume. We investigated P450 2B4 - benzphetamine or P450scc - cholesterol enzyme - substrate pairs. Electrochemical parameters of electrodes with nonspecific P450 substrate were differed from electrodes with appropriate substrates.
Download PDF:  
Keywords: cytochrome P450, electrochemistry, screen-printed electrodes, cholesterol, electrocatalysis

Shumyantseva, V. V., Bulko, T. V., Kuznetsova, G. P., Samenkova, N. F., Bachman, T., Schulze, H., Schmid, R. D., Usanov, S. A., Archakov, A. I. (2006). Electrochemical reduction of cytochrome P450s based on electrodes with immobilized substrates. Biomeditsinskaya Khimiya, 52(1), 44-51.
This paper is also available as the English translation: 10.1134/S1990750807010118
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)