Biodegradable microparticles with immobilized peptide for wound healing

Stashevskaya K.S.1 , Markvicheva E.A.1, Strukova S.M.2, Rusanova A.V.2, Makarova A.M.2, Gorbacheva L.R.2, Prudchenko I.A.1, Zubov V.P.1, Grandfis K.3

1. M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemisty, Russian Academy of Sciences
2. School of Biology, Moscow State University
3. Liege University
Section: Experimental/Clinical Study
PubMed Id: 16739924
Year: 2006  Volume: 52  Issue: 1  Pages: 83-94
Thrombin receptor agonist peptide (TRAP-6) may effectively replace thrombin for stimulation of damaged tissue regeneration. (Thrombin employment is limited by its high cost, instability and proinflammatory effect at high concentrations.) Immobilization of TRAP-6 into a poly(D,L)-lactide-co-glycolide (PLGA)-based matrix can protect peptides from a destruction by peptidases located in a wound area, and can also provide controlled release of the peptide.PLGA microparticles with immobilized peptide were produced by double emulsion/evaporation technique. An observation of microparticle morphology by scanning electron microscopy highlighted that peptide immobilization resulted in the increase of the microparticle porosity. TRAP-6 release kinetics was characterized by burst increase of TRAP-6 concentration in HEPES buffer solution (рН 7.5) for first 2 hours from the beginning of the experiment, and TRAP-6 complete release occurred for 20 hours. An investigation of TRAP-6 destruction by scanning electron microscopy revealed that the increase of microparticle size and surface porosity were observed already after 1 day of incubation in the buffer solution, and an aggregation of destructing microparticles was obvious by the 7th day of the incubation.Thus, peptide immobilization into PLGA microparticles can allow to develop a novel controlled release drug delivery system.
Download PDF:  
Keywords: immobilization, TRAP-6, PLGA microparticles, controlled release

Stashevskaya, K. S., Markvicheva, E. A., Strukova, S. M., Rusanova, A. V., Makarova, A. M., Gorbacheva, L. R., Prudchenko, I. A., Zubov, V. P., Grandfis, K. (2006). Biodegradable microparticles with immobilized peptide for wound healing. Biomeditsinskaya Khimiya, 52(1), 83-94.
This paper is also available as the English translation: 10.1134/S1990750807020072
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)