Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-N-oxide derivatives

   
Volkova T.O.1 , Zykina N.S.1, Malycheva I.E.1, Nemova N.N.1

1. Department of Molecular Biology, Petrozavodsk State University
Section: Experimental/Clinical Study
PubMed Id: 16805390
Year: 2006  Volume: 52  Issue: 2  Pages: 180-187
We have studied the influence of 2-(4'-nitrostyryl)-quinoline-1-oxide (2-NSQO) and 4-(4'-nitrostyryl)-quinoline-1-oxide (4-NSQO) on modulation activity of microsomal NADPH-oxidoreductases, concentration of nicotinamide enzymes and induction of apoptosis in human erythroleukaemic K562 cells. It was shown, that activity of microsomal NADPH-cytochrome c-reductases in cancer cells was inhibited by 10 μM 4-NSQO (15%), and 10 μM 2-NSQO (50%). Treatment of cells with these reagents for two days, was accompanied by caspases-9 and -3 activation, rise of EtBr and DAPI fluorescence related to DNA binding and induction of apoptosis. Apoptosis was preceded by the decrease of concentration of nicotinamide enzymes. Thus 4-NSQO is a promising compound for further experimental trials as an anticancer drug with low toxic action on the tissues of organism.
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Keywords: cancer cell lines, apoptosis, caspases, NADPH-dependent reductases, quinoline-N-oxide derivatives
Citation:

Volkova, T. O., Zykina, N. S., Malycheva, I. E., Nemova, N. N. (2006). Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-N-oxide derivatives. Biomeditsinskaya khimiya, 52(2), 180-187.
This paper is also available as the English translation: 10.1134/S199075080701012X
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