We have studied the influence of 2-(4'-nitrostyryl)-quinoline-1-oxide (2-NSQO) and 4-(4'-nitrostyryl)-quinoline-1-oxide (4-NSQO) on modulation activity of microsomal NADPH-oxidoreductases, concentration of nicotinamide enzymes and induction of apoptosis in human erythroleukaemic K562 cells. It was shown, that activity of microsomal NADPH-cytochrome c-reductases in cancer cells was inhibited by 10 μM 4-NSQO (15%), and 10 μM 2-NSQO (50%). Treatment of cells with these reagents for two days, was accompanied by caspases-9 and -3 activation, rise of EtBr and DAPI fluorescence related to DNA binding and induction of apoptosis. Apoptosis was preceded by the decrease of concentration of nicotinamide enzymes. Thus 4-NSQO is a promising compound for further experimental trials as an anticancer drug with low toxic action on the tissues of organism.
Download PDF:
Keywords: cancer cell lines, apoptosis, caspases, NADPH-dependent reductases, quinoline-N-oxide derivatives
Citation:
Volkova T.O., Zykina N.S., Malycheva I.E., Nemova N.N. (2006) Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-N-oxide derivatives. Biomeditsinskaya Khimiya, 52(2), 180-187.
Volkova T.O. et al. Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-N-oxide derivatives // Biomeditsinskaya Khimiya. - 2006. - V. 52. -N 2. - P. 180-187.
Volkova T.O. et al., "Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-N-oxide derivatives." Biomeditsinskaya Khimiya 52.2 (2006): 180-187.
Volkova, T. O., Zykina, N. S., Malycheva, I. E., Nemova, N. N. (2006). Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-N-oxide derivatives. Biomeditsinskaya Khimiya, 52(2), 180-187.
