Isoelectrofocusing andphotocolorimetry fordeterminationofglycatedhemoglobin (HbA1c)

   
Korolev V.A.1 , Molchanov V.I.1

1. Crimea State Medical University
Section: Experimental/Clinical Study
UDK: 616.155.16 615.543.545-073      PubMed Id: 16805393
Year: 2006  Volume: 52  Issue: 2  Pages: 200-210
Analytical reliability of a new method for determination of glycated hemoglobin (HbA1c) has been evaluated. The method is based on hemoglobin isolation during isoelectrofocusing followed by acidic hydrolysis and photocolorimetry of furfurol formed after its reaction with thiobarbituric acid. At diabetic and non-diabetic levels inter- and intraserial reproducibility varied from 6 to 21%. Sensitivity of this method was determined using physiological solution and 50% albumin.
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Korolev V.A., Molchanov V.I. (2006) Biomeditsinskaya khimiya, 52(2), 200-210.
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