Oligomerization of L-asparaginase from Erwinia carotovora

Mezentsev Y.V.1, Molnar A.A.1, Gnedenko O.V.1, Krasotkina Y.V.1, Sokolov N.N.1, Ivanov A.S.1

1. V.N.Orekhovich Institute of Biomedical Chemistry RAMS
Section: Experimental/Clinical Study
PubMed Id: 16898584
Year: 2006  Volume: 52  Issue: 3  Pages: 258-271
Bacterial L-asparaginases catalyzing hydrolysis of L-asparagine up to L-aspartate and ammonia, are used in medical practice for treatment of acute lymphoblastic leukemia. The long-term therapy with these preparations is accompanied by a number of side effects, which are attributed to glutaminase activity of L-asparaginase. Substrate specificity and activity of L-asparaginases are directly associated with the process of enzyme oligomerization. It is active only in the tetrameric form as the active sites are located in contact areas between monomers. The present work is devoted to homology modeling of spatial structure of L-asparaginase from Erwinia carotovora, the comparative molecular-graphic analysis of subunits interfaces, as well as development of experimental approach for enzyme oligomerization study. L-asparaginase was immobilized on a CM5 chip surface of optical biosensor Biacore 3000 based on the surface plasmon resonance technology. The dissociation process of enzyme tetrameric complexes up to monomers and subsequent oligomerization process have been registered.
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Keywords: oligomerization, L-asparaginase, Erwinia carotovora, Erwinia chrysanthemi, computer modeling, optical biosensor Biacore 3000

Mezentsev, Y. V., Molnar, A. A., Gnedenko, O. V., Krasotkina, Y. V., Sokolov, N. N., Ivanov, A. S. (2006). Oligomerization of L-asparaginase from Erwinia carotovora. Biomeditsinskaya Khimiya, 52(3), 258-271.
This paper is also available as the English translation: 10.1134/S199075080701009X
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