Nitric oxide synthase activity and nitrates/nitrites level in brain regions during the spontaneous morphine withdrawal in rats

Peregud D.I.1 , Onufriev M.V.2, Yakovlev A.A.3, Stepanichev M.Y.2, Lazareva N.A.2, Pavlova T.V.2, Panchenko L.F.1, Gulyaeva N.V.2

1. National Scientific Center on Addiction, Ministry of Healthcare of RF
2. Institute of Higher Nervous Activity and Neurophysiology RAS
3. Institute of Higher Nervous Activity and Neurophysiology RAS Moscow Institute of Physics and Technology
Section: Experimental/Clinical Study
UDK: 615.015.6+612.82      PubMed Id: 17044593
Year: 2006  Volume: 52  Issue: 4  Pages: 352-363
Nitric oxide synthase (NOS) activity and nitrate/nitrites (NOx-) concentrations were measured in brain regions of rats during the spontaneous morphine withdrawal. Male Wistar rats were injected intraperotoneally with morphine hydrochloride at increasing doses (10-100 mg/kg) during 6 days twice a day. Thirty six hours after the last injection the severity of the spontaneous morphine withdrawal syndrome was determined by specific autonomic and locomotor indices. Both NOS activity and NOx- levels increased in the midbrain and the hippocampus, decreased in the striatum and the hypothalamus, and did not change in the cerebral cortex and the brain stem. NOx- concentrations in the cerebellum did not change, while NOS activity decreased. Both NOS activities and NOx- concentrations in the cerebral cortex, striatum, midbrain, and cerebellum correlated with withdrawal syndrome severity on the whole, and with the specific signs of abstinence.
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Peregud D.I., Onufriev M.V., Yakovlev A.A., Stepanichev M.Y., Lazareva N.A., Pavlova T.V., Panchenko L.F., Gulyaeva N.V. (2006) Biomeditsinskaya khimiya, 52(4), 352-363.
This paper is also available as the English translation:10.1134/S1990750807020047
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