Nanoelectrochemistry of cytochrome P450s: direct electron transfer and electrocatalysis

Shumyantseva V.V.1 , Bulko T.V.1, Rudakov Yu.O.1, Kuznetsova G.P.1, Samenkova N.F.1, Lisitsa A.V.1, Karuzina I.I.1, Archakov A.I.1

1. Insitute of Biomedical Chemistry, Russian Academy of Medical Sciences
Section: Experimental/Clinical Study
PubMed Id: 17180920
Year: 2006  Volume: 52  Issue: 5  Pages: 458-468
The present study demonstrates the direct electron transfer between cytochrome P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14α-demethylase (CYP51MT) and screen printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB). Electrodetection of heme proteins is possible when 2-200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, lanosterol) and inhibitor ketoconazole were analyzed using cyclic voltammetry (CV), square wave (SWV) or differential pulse (DPV) voltammetry, amperometry.
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Keywords: cytochrome P450 2B4, P450 1A2, sterol 14α-demethylase from Mycobacterium tuberculosis (CYP51MT), lanosterol, ketoconazole, screen printed electrodes, bioelectrochemistry, gold nanoparticles, nanostructured electrodes

Shumyantseva, V. V., Bulko, T. V., Rudakov, Yu. O., Kuznetsova, G. P., Samenkova, N. F., Lisitsa, A. V., Karuzina, I. I., Archakov, A. I. (2006). Nanoelectrochemistry of cytochrome P450s: direct electron transfer and electrocatalysis. Biomeditsinskaya khimiya, 52(5), 458-468.
This paper is also available as the English translation: 10.1134/S1990750807040075
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