Bioluminescent method for determination of concentration metabolic substrates and NAD+ in the lymphocytes

   
Fomenko E.J.1, Slepov E.V.2 , Inzhevatkin E.V.3 , Savchenko A.A.2

1. Department of Human and Animal Biochemistry and Physiology, Krasnoyarsk State University
2. Laboratory of Cell and Molecular Physiology and Pathology, Scientific Research Institute of Medical Problems of North, Siberian Division of Russian Academy of Medical Sciences
3. International Scientific Center for Organism Extreme State Research attached to Presidium of Krasnoyarsk Scientific Center, Siberian Division of Russian Academy of Sciences
Section: Experimental/Clinical Study
PubMed Id: 17180925
Year: 2006  Volume: 52  Issue: 5  Pages: 507-510
We have developed a new bioluminescent method for determination of concentrations of pyruvate, lactate, malate, glutamate and NAD+ in the periferal blood lymphocytes from mice with Ehrlich ascites carcinoma. There were blood samples of intact mice for control. We found that variations of these substrates, depend on stage of malignant growth.
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Keywords: lymphocytes, pyruvate, lactate, malate, glutamate, NAD+, bioluminescent approach
Citation:

Fomenko, E. J., Slepov, E. V., Inzhevatkin, E. V., Savchenko, A. A. (2006). Bioluminescent method for determination of concentration metabolic substrates and NAD+ in the lymphocytes. Biomeditsinskaya Khimiya, 52(5), 507-510.
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