Influence of tryptophan content in proteins on the quantitative determination of this proteins using photochemical p-dimethylaminobenzaldehyde

   
Nikitenko E.A.1, Zaitsev V.G.1, Ostrovskij O.V.1

1. Department of Basic and Clinical Biochemistry, Volgograd State Medical University
Section: Experimental/Clinical Study
UDK: 543.422.7:577.112.825`34`387.4      PubMed Id: 17639724
Year: 2007  Volume: 53  Issue: 2  Pages: 216-220
The interaction of proteins containing various number of Trp residues with p-dimethylamino- benzaldehyde (pDABA) was studied. A positive correlation between tryptophan content in proteins and absorbance of protein-pDABA reaction products was found (Spearman`s correlation coefficient R=0,936; p<0,001). Using the observed correlation we were able to predict a possible range for the absorbance of reaction products for each serum globulin and estimate the contribution of different globulins in the pDABA serum assay. We propose that the described method can be used for the determination of the globulin content in blood serum.
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Nikitenko E.A., Zaitsev V.G., Ostrovskij O.V. (2007) Biomeditsinskaya khimiya, 53(2), 216-220.
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