Results of experiments on modification of immunostimulating complexes (ISCOM`s) matrix by the replacement of the phospholipid for the glycolipid (monogalactosyldiacylglycerol) from sea macrophytes, and saponin QuillA to triterpene glycoside of сuсumarioside A2-2 from Cucumaria japonica are shown. The resultant complexes include the morphological structures of two types: ISCOM-like structures with the characteristic morphology and sizes and also the tubular structures with diameter of approximately 40 nm and length of 150-400 nm. We have named these structures as TI-complexes. These TI-complexes exhibit considerably lower toxicity than ISCOM. They may include an amphiphilic protein antigen and provide immunoadjuvant effect during experimental vaccination. Under conditions of experimental immunization of mice by a weak immunogen - (subunit membrane pore protein from Y. pseudotuberculosis), TI-complexes with antigen provided stronger humoral immune response to antigen than the complexes of porin with classical ISCOM, liposomes and Freund`s adjuvant. Thus, it`s shown the prospect of the use of TI-complexes as a new type of adjuvant carriers for antigens.
Keywords: adjuvant, ISCOM, triterpene glycoside, monogalactosyldiacylglycerol (MGDG), vaccine, porin
Tsybulsky A.V. et al. Elaboration of new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen // Biomeditsinskaya khimiya. - 2007. - V. 53. -N 3. - P. 297-306.
Tsybulsky A.V. et al., "Elaboration of new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen." Biomeditsinskaya khimiya 53.3 (2007): 297-306.
Tsybulsky, A. V., Sanina, N. M., Lee, I. A., Popov, A. M., Kostetsky, E. Ja., Portnyagina, O. Y., Shnyrov, V. L. (2007). Elaboration of new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen. Biomeditsinskaya khimiya, 53(3), 297-306.