Biochemical and photometric studies of modification of collagen structure under UV radiation

   
Rebrova G.A.1 , Vasilevsky V.K.1, Rebrov L.B.1, Osipova L.A.1, Bykov V.A.1

1. Scientific Research and Eduction Centre of Biomedical Technology
Section: Experimental/Clinical Study
PubMed Id: 18035725
Year: 2007  Volume: 53  Issue: 4  Pages: 442-453
The influence of UV irradiation (270-380 nm) on the biochemical, fluorescence and colorimetric properties of collagen was studied. The long-term UV irradiation (120 h) was accompanied by the increase of structural stability of collagen to specific and nonspecific proteolytic enzymes, by formation of new additional fluorophore-containing molecules, by the increase in quantity of carbonyl groups in the collagen and by strong changing of the distribution pattern of alkaline hydrolysis products during gel-chromatography. The coordinates of colour of the collagen films are also changed. These changes of collagen suggest that UV irradiation induces photomodification and photooxidation processes in collagen.
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Keywords: collagen, UV radiation, photochemistry, photoaging
Citation:

Rebrova, G. A., Vasilevsky, V. K., Rebrov, L. B., Osipova, L. A., Bykov, V. A. (2007). Biochemical and photometric studies of modification of collagen structure under UV radiation. Biomeditsinskaya Khimiya, 53(4), 442-453.
This paper is also available as the English translation: 10.1134/S1990750807040129
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