Expression of matrix metalloproteinase (MMP-2) and extracellular matrix metalloproteinases inducer (EMMPRIN) in benign and advanced breast cancer tissue samples

   
Chandru H.1 , Sharada A.C.1, Manjunath S.2

1. Department of Biochemistry, Yuvaraja`s college, University of Mysore
2. Department of Studies in Biochemistry, Manasagangotri, University of Mysore
Section: Experimental/Clinical Study
PubMed Id: 18035727
Year: 2007  Volume: 53  Issue: 4  Pages: 461-467
Tumor cell derived matrix metalloproteinases are a family of enzymes associated with the tumor invasion and metastasis. Extracellular matrix metalloproteinases inducer (EMMPRIN) stimulates synthesis of gelatinase A (MMP-2) in peritoneal fibroblasts. In the present study the role of MMP-2 and EMMPRIN in the progression of breast cancer has been investigated. Gelatinase-A and EMMPRIN were analyzed in benign and stage II and stage III breast cancer tissue samples by gelatin zymography assay, immunoprecipation analysis and Western blot analysis with a monoclonal primary antibody specific for EMMPRIN. Our results showed overexpression of EMMPRIN in advanced stages of breast cancer tissues compared with benign tumor tissue samples. The expression of MMP-2, the active and latent forms of the enzyme increased with tumor progression from Stage II to Stage III of breast cancer and it was not expressed in benign tissues. The expression MMP-2 correlates with tumor progression. This observation obviously indicates that EMMPRIN and MMP-2 are the major determinants of malignancy in cancers.
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Keywords: EMMPRIN, MMP-2, tumor invasion, breast cancer, Western blot
Citation:

Chandru, H., Sharada, A. C., Manjunath, S. (2007). Expression of matrix metalloproteinase (MMP-2) and extracellular matrix metalloproteinases inducer (EMMPRIN) in benign and advanced breast cancer tissue samples. Biomeditsinskaya khimiya, 53(4), 461-467.
This paper is also available as the English translation: 10.1134/S1990750807040105
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