Methods of quantitative proteomics

   
Kopylov A.T.1 , Zgoda V.G.1

1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
Section: Experimental/Clinical Study
PubMed Id: 18323148
Year: 2007  Volume: 53  Issue: 6  Pages: 613-643
In modern science proteomic analysis is inseparable from other fields of systemic biology. Possessing huge resources quantitative proteomics operates colossal information on molecular mechanisms of life. Advances in proteomics help researchers to solve complex problems of cell signaling, posttranslational modification, structure and functional homology of proteins, molecular diagnostics etc. More than 40 various methods have been developed in proteomics for quantitative analysis of proteins. Although each method is unique and has certain advantages and disadvantages all these use various isotope labels (tags). In this review we will consider the most popular and effective methods employing both chemical modifications of proteins and also metabolic and enzymatic methods of isotope labeling.
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Keywords: quantitative proteomics, mass spectrometry, isotope label, chemical modification
Citation:

Kopylov, A. T., Zgoda, V. G. (2007). Methods of quantitative proteomics. Biomeditsinskaya Khimiya, 53(6), 613-643.
This paper is also available as the English translation: 10.1007/s11828-008-1003-2
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