Spreading and mechanisms of antimicrobial resistance of micro-organisms, producing beta-lactamases. Phenotypical screening mbl producers (carbapenemases b1) among strains of Pseudomonas genus, isolated in cases of nosocomial infections
Intrahospital strains (215) of bacterial genus Pseudomonas isolated from patients of 30 Medical centers of 15 Russian regions have been investigated for antibiotic resistance. The bacterial cultures resistant to imipenem and/or meropenem were considered as metallo-beta-lactamase (MBL) producers. Production of subclass B1 MBL (carbapenemases) was evaluated by means of the double-disk approximation test using MBL inhibitor, EDTA. There were 55 P. aeroginosa strains (25.6%) resistant to imipenem and meropenem simultaneously; 19 isolates (8.8%) of P. aeroginosa were characterized by synergism between carbapenem and EDTA. The subclass B1 MBL producers are widely distributed in the intrahospital strain obtained from Moscow, Yaroslavl, Ekaterinburg, Omsk, and Tomsk hospitals.
Download PDF:
Keywords: intrahospital infections, Pseudomonas spp., antibiotic resistance, phenotyping, screening, blue pus rod bacterium, metallo-beta-lactamases
Citation:
Ivanov D.V., Egorov A.M. (2007) Spreading and mechanisms of antimicrobial resistance of micro-organisms, producing beta-lactamases. Phenotypical screening mbl producers (carbapenemases b1) among strains of Pseudomonas genus, isolated in cases of nosocomial infections. Biomeditsinskaya Khimiya, 53(6), 653-661.
Ivanov D.V. et al. Spreading and mechanisms of antimicrobial resistance of micro-organisms, producing beta-lactamases. Phenotypical screening mbl producers (carbapenemases b1) among strains of Pseudomonas genus, isolated in cases of nosocomial infections // Biomeditsinskaya Khimiya. - 2007. - V. 53. -N 6. - P. 653-661.
Ivanov D.V. et al., "Spreading and mechanisms of antimicrobial resistance of micro-organisms, producing beta-lactamases. Phenotypical screening mbl producers (carbapenemases b1) among strains of Pseudomonas genus, isolated in cases of nosocomial infections." Biomeditsinskaya Khimiya 53.6 (2007): 653-661.
Ivanov, D. V., Egorov, A. M. (2007). Spreading and mechanisms of antimicrobial resistance of micro-organisms, producing beta-lactamases. Phenotypical screening mbl producers (carbapenemases b1) among strains of Pseudomonas genus, isolated in cases of nosocomial infections. Biomeditsinskaya Khimiya, 53(6), 653-661.
Jones R., Biedenbach D., Sader H., Eristshe T., Toleman M., Walsh R. (2005) Diagn. Microbiol. Infect. Dis., 51, 77-84. Scholar google search
Jones R., Deshpande L., Bell J., Tunidge J., Kohno S., Hirakata Y., Ono Y., Miyzawa Y., Kawakama S., Inoue M., Hizata Y., Toleman M. (2004) Diagn. Microbiol. Infect. Dis., 49, 289-294. Scholar google search
