Ribosomal repeat in the cell free DNA as a marker for cell death

Veiko N.N.1 , Bulycheva N.A.1, Roginko O.A.2, Veiko R.V.1, Ershova E.S.1, Kozdoba O.A.2, Kuzmin V.A.3, Vinogradov A.M.3, Yudin A.A.2, Speranskyi A.I.4

1. Research Centre for Medical Genetics, Russian Academy of Medical Sciences
2. Central clinical Russian Academy of Sciences
3. Institute of Biochemical Physics, Russian Academy of Sciences
4. Institute of Rheumatology of Russian Academy of Medical Sciences
Section: Experimental/Clinical Study
PubMed Id: 18421913
Year: 2008  Volume: 54  Issue: 1  Pages: 78-93
We have developed a novel method for in vivo evaluation of cell death in patients with acute and/or chronic heart diseases, which are accompanied by apoptosis or cell necrosis. The method is based on the analysis of cell free DNA (cfDNA) in the blood serum (or plasma). The major parameters assessed in the method include total concentration of serum cfDNA, concentration of serum ribosomal repeat (rDNA), content of rDNA in total cfDNA, as well as factors of cfDNA elimination, such as nuclease activity and anti-DNA antibody. We demonstrated a fivefold increase in the serum cfDNA concentration and a 12-fold enhancement of serum rDNA concentration in patients with acute myocardial infarction compared with healthy individuals. In chronic coronary ischemia the serum cfDNA concentration was similar to that in the disease-free group. However, the content of rDNA in cfDNA was 4.8-fold higher, and the serum rDNA concentration was increased sevenfold. We hypothesize that one reason for accumulation of rDNA within cfDNA might be the previously reported resistance of rDNA to the ds-fragmentation by serum endonucleases. In both acute and chronic coronary disease the nuclease activity in the serum was substantially higher than that in the healthy cohort. Moreover, the titer of anti-DNA antibodies was elevated, with these antibodies being mostly bound to the cfDNA. Thus, the release of rDNA fragments into the blood not only reflects cellular death in the body but also determines the response of the organism to the disease-associated stress.
Download PDF:  
Keywords: cell free DNA, ribosomal genes, endonuclease activity, acute myocardial infarction, cardiac ischemia

Veiko, N. N., Bulycheva, N. A., Roginko, O. A., Veiko, R. V., Ershova, E. S., Kozdoba, O. A., Kuzmin, V. A., Vinogradov, A. M., Yudin, A. A., Speranskyi, A. I. (2008). Ribosomal repeat in the cell free DNA as a marker for cell death. Biomeditsinskaya Khimiya, 54(1), 78-93.
This paper is also available as the English translation: 10.1134/S1990750808020121
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)