Comparative transcriptomic and proteomic analysis of fetal and adult human liver

Kurbatov L.K.1 , Cheglakov I.B.1, Yarygin K.N.1, Sukhikh G.T.2, Vartanian G.V.3, Toropygin I.Y.1, Archakov A.I.1

1. Institute of Biomedical Chemistry Russian Academy of Medical Sciences
2. Research Centre for Obstetrics, Gynaecology and Perinatology Russian Academy of Medical Sciences
3. Russian Scientific Surgery Centre of Russian Academy of Medical Sciences
Section: Experimental/Clinical Study
PubMed Id: 18522216
Year: 2008  Volume: 54  Issue: 2  Pages: 140-153
The transcriptome and proteome comparative analysis of the fetal liver (9.5-10.5 weeks of gestation) and normal adult liver was developed in the present investigation. We used 44k microarrays of Agilent company and 2D-GE MALDI-TOF in transcriptome and proteome approaches, respectively. The top lists of expression genes and proteins for fetal liver obtained by these methods were compared. Transcriptome analysis confirmed proteome data only partially, but these two semi-quantitative approaches established the interdependences between expression levels of genes and proteins. The discrepancies between data obtained by two approaches were discussed.The unique feature of the fetal liver cell content is the combination of hemopoietic and nonmaturated liver cells. Fetal liver changes its hemopoietic role during embryogenesis towards the detoxicative tissue being able to metabolize different substances and produce wide serum of proteins. The status of fetal liver as the hemopoietic tissue was entirely characterized by transcriptome analysis having registered embryonic and adult hemoglobins, insufficient cytochrome-dependent detoxification system and highly developed anti-apoptotic potential.
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Keywords: two-dimensional electrophoresis, expression analysis, DNA microarrays, gene expression, fetal liver

Kurbatov, L. K., Cheglakov, I. B., Yarygin, K. N., Sukhikh, G. T., Vartanian, G. V., Toropygin, I. Y., Archakov, A. I. (2008). Comparative transcriptomic and proteomic analysis of fetal and adult human liver. Biomeditsinskaya Khimiya, 54(2), 140-153.
This paper is also available as the English translation: 10.1134/S1990750808030025
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