Peptide RHDSGY that represents the fragment of human β-amyloid Zn-binding site and its isomers RH(D-Asp)SGY and RH(β-Asp)SGY have been prepared by solid-phase synthesis and analysed by HPLC and various mass-spectrometric methods. The problem of low yield of peptide RHDSGY and its isomers attributed to 9-fluorenylmethoxycarbonyl (Fmoc)-amino acids and/or formation of side-products as RH(Asp-imide)SGY and RHDSGY (instead of RH(β-Asp)SGY) was solved via selection of reagents for the removal of Fmoc groups from the growing peptide chain.
Aleshina E.Yu., Pyndyk N.V., Moisa A.A., Sanzhakov M.A., Kharybin O.N., Nikolaev E.N., Kolesanova E.F. (2008) Synthesis of β-amyloid fragment 5RHDSGY10 and its isomers with the use of new solid-phase peptide synthesis schedule. Biomeditsinskaya Khimiya, 54(2), 184-191.
Aleshina E.Yu. et al. Synthesis of β-amyloid fragment 5RHDSGY10 and its isomers with the use of new solid-phase peptide synthesis schedule // Biomeditsinskaya Khimiya. - 2008. - V. 54. -N 2. - P. 184-191.
Aleshina E.Yu. et al., "Synthesis of β-amyloid fragment 5RHDSGY10 and its isomers with the use of new solid-phase peptide synthesis schedule." Biomeditsinskaya Khimiya 54.2 (2008): 184-191.
Aleshina, E. Yu., Pyndyk, N. V., Moisa, A. A., Sanzhakov, M. A., Kharybin, O. N., Nikolaev, E. N., Kolesanova, E. F. (2008). Synthesis of β-amyloid fragment 5RHDSGY10 and its isomers with the use of new solid-phase peptide synthesis schedule. Biomeditsinskaya Khimiya, 54(2), 184-191.
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