Synthesis of β-amyloid fragment 5RHDSGY10 and its isomers with the use of new solid-phase peptide synthesis schedule

   
Aleshina E.Yu.1 , Pyndyk N.V.1, Moisa A.A.1, Sanzhakov M.A.1, Kharybin O.N.1, Nikolaev E.N.1, Kolesanova E.F.1

1. Orekhovich Institute of Biomedical Chemistry, Russian Аcademy of Medical Sciences
Section: Experimental/Clinical Study
UDK: 577.112.6      PubMed Id: 18522220
Year: 2008  Volume: 54  Issue: 2  Pages: 184-191
Peptide RHDSGY that represents the fragment of human β-amyloid Zn-binding site and its isomers RH(D-Asp)SGY and RH(β-Asp)SGY have been prepared by solid-phase synthesis and analysed by HPLC and various mass-spectrometric methods. The problem of low yield of peptide RHDSGY and its isomers attributed to 9-fluorenylmethoxycarbonyl (Fmoc)-amino acids and/or formation of side-products as RH(Asp-imide)SGY and RHDSGY (instead of RH(β-Asp)SGY) was solved via selection of reagents for the removal of Fmoc groups from the growing peptide chain.
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Aleshina E.Yu., Pyndyk N.V., Moisa A.A., Sanzhakov M.A., Kharybin O.N., Nikolaev E.N., Kolesanova E.F. (2008) Biomeditsinskaya khimiya, 54(2), 184-191.
This paper is also available as the English translation:10.1134/S1990750808030098
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