Determination of proteases activity of blood and microorganisms

Kozlov L.V.1 , Bichucher A.M.1, Mishin A.A.1, D'yakov V.L.1, Leont'eva N.I.1, Gracheva N.M.1, Novikova L.I.1

1. Gabrichevsky Moscow Institute of Microbiology and Epidemiology
Section: Experimental/Clinical Study
PubMed Id: 18712087
Year: 2008  Volume: 54  Issue: 3  Pages: 314-321
For determination of protease activity it is possible to use immunoglobulins. Since proteolytic products apparently do not retain substrate antigenic determinants, it is possible to use ELISA methodsfor monitoring for enzymatic process . ELISA determination of functional activity of specific IgA1-protease has been applied not only for detection of this enzyme, but also for measurement of its inhibition constants. Fixed on a micropanel IgG may be used for evaluation of total proteolytic activity. Depending on pH values, it is possible to measure activity of neutral, alkaline and acid proteases. This approach has allowed to estimate total proteolytic activity of neutral proteases of serum. Measurement of a total level of serum pepsinogene activity can have diagnostic importance in gastroenterology, due to decisive contribution of pepsinogen I to the measured activity.
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Keywords: proteases, immunoglobulins, IgA1-protease, activity, inhibition, ELISA

Kozlov, L. V., Bichucher, A. M., Mishin, A. A., D'yakov, V. L., Leont'eva, N. I., Gracheva, N. M., Novikova, L. I. (2008). Determination of proteases activity of blood and microorganisms. Biomeditsinskaya Khimiya, 54(3), 314-321.
This paper is also available as the English translation: 10.1134/S1990750808040082
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