1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences 2. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
Using reverse-phase (MB-HIC 8 and HB-HIC 18) weak cation exchange (MB-WCX) and metal affinity ClinProt magnetoc beads peptides and protein factions were obtained from human sera for their profiling by MALDI-TOF mass spectrometry. Proteome profiling of sera from I-IV stage ovarian cancer patients (47 women, average age 51) and from healthy women (47 subjects, average age 49) using MB-WCX beads allowed calculation of the best diagnostic models based on the Genetic Algorithm and Supervised Neural Network classifiers; these model generated 100% sensitivity and specificity when the test set of subjects was analyzed. Introduction of additional sera from patients with colorectal cancer (19) and ulcerous colitis (5) to the statistical model confirmed 100% ovarian cancer recognition. Statistical mass-spectrometry analysis of mass-spectrometry peak areas included to the diagnostic classifiers showed 3 peaks distinctive for ovarian cancer and 4 peaks distinctive for ovarian and colorectal cancer.
Download PDF:
Keywords: proteome profiling, serum, MALDI-TOF-mass-spectrometry, ClinProt magnetic beads, ovarian cancer
Citation:
Ziganshin R.H., Alexeev D.G., Arapidi G.P., Ivanov V.T., Moshkovskii S.A., Govorun V.M. (2008) Serum proteome profiling for ovarion cancer diagnosis using ClinProt magnetic bead technique and MALDI-TOF-mass-spectrometry. Biomeditsinskaya Khimiya, 54(4), 408-419.
Ziganshin R.H. et al. Serum proteome profiling for ovarion cancer diagnosis using ClinProt magnetic bead technique and MALDI-TOF-mass-spectrometry // Biomeditsinskaya Khimiya. - 2008. - V. 54. -N 4. - P. 408-419.
Ziganshin R.H. et al., "Serum proteome profiling for ovarion cancer diagnosis using ClinProt magnetic bead technique and MALDI-TOF-mass-spectrometry." Biomeditsinskaya Khimiya 54.4 (2008): 408-419.
Ziganshin, R. H., Alexeev, D. G., Arapidi, G. P., Ivanov, V. T., Moshkovskii, S. A., Govorun, V. M. (2008). Serum proteome profiling for ovarion cancer diagnosis using ClinProt magnetic bead technique and MALDI-TOF-mass-spectrometry. Biomeditsinskaya Khimiya, 54(4), 408-419.
Аксель Е.М., Давыдов М.И. (2002) Сборник `Злокачественные новообразования в России и странах СНГ в 2000`, РОНЦ им. Н.Н. Блохина РАМН, Москва. Scholar google search
Posadas E.M., Simpkins F., Liotta L.A., MacDonald C., Kohn E.C. (2005) Ann. Oncol., 16, 16-22. Scholar google search
Bidart J.M., Thuillier F., Augereau C., Chalas J., Daver A., Jacob N., Labrousse F., Voitot H. (1999) Clin Chem., 45, 1695-1707. Scholar google search
Mor G., Visintin I., Lai Y., Zhao H., Schwartz P., Rutherford T., Yue L., Bray-Ward P., Ward D. C. (2005) Proc. Natl. Acad. Sci. USA, 102, 7677-7682. Scholar google search
Anderson N. L., Anderson N. G. (2002) Mol. Cell Proteomics, 1, 845-867. Scholar google search
Merchant M., Weinberger S.R. (2000) Electrophoresis, 21, 1164-1177. Scholar google search
Su Y., Shen J., Qian H., Ma H., Ji J., Ma L., Zhang W., Meng L., Li Z., Wu J., Jin G., Zhang J., Shou C. (2007) Cancer Sci., 98, 37-43. Scholar google search
Smith F.M., Gallagher W.M., Fox E., Stephens R.B., Rexhepaj E., Petricoin E.F., 3rd, Liotta L., Kennedy M.J., Reynolds J.V. (2007) Ann. Surg., 245, 259-266. Scholar google search
Skytt A., Thysell E., Stattin P., Stenman U.H., Antti H., Wikstrom P. (2007) Int. J. Cancer, 121, 615-620. Scholar google search
Kanmura S., Uto H., Kusumoto K., Ishida Y., Hasuike S., Nagata K., Hayashi K., Ido A., Stuver S.O., Tsubouchi H. (2007) Hepatology, 45, 948-956. Scholar google search
Li J., Zhang Z., Rosenzweig J., Wang Y.Y., Chan D.W. (2002) Clin. Chem., 48, 1296-1304. Scholar google search
Zhou L., Cheng L., Tao L., Jia X., Lu Y., Liao P. (2006) Acta Otolaryngol., 126, 853-860. Scholar google search
Zheng G.X., Wang C.X., Qu X., Deng X.M., Deng B.P., Zhang J. (2006) Exp. Oncol., 28, 282-287. Scholar google search
Zhang H., Kong B., Qu X., Jia L., Deng B., Yang Q. (2006) Gynecol. Oncol., 102, 61-66. Scholar google search
Wang J.X., Yu J.K., Wang L., Liu Q.L., Zhang J., Zheng S. (2006) Proteomics, 6, 5344-5349. Scholar google search
de Noo M.E., Deelder A., van der Werff M., Ozalp A., Mertens B., Tollenaar R. (2006) Onkologie, 29, 501-506. Scholar google search
Ebert M.P., Niemeyer D., Deininger S.O., Wex T., Knippig C., Hoffmann J., Sauer J., Albrecht W., Malfertheiner P., Rocken C. (2006) J. Proteome Res., 5, 2152-2158. Scholar google search
Shin S., Cazares L., Schneider H., Mitchell S., Laronga C., Semmes O.J., Perry R.R., Drake R.R. (2007) J. Am. Coll. Surg., 204, 1065-1071. Scholar google search
Villanueva J., Philip J., Entenberg D., Chaparro C.A., Tanwar M.K., Holland E.C., Tempst P. (2004) Anal. Chem., 76, 1560-1570. CrossRef Scholar google search
Villanueva J., Philip J., Chaparro C.A., Li Y., Toledo-Crow R., DeNoyer L., Fleisher M., Robbins R.J., Tempst P. (2005) J. Proteome Res., 4, 1060-1072. Scholar google search
Lopez M.F., Mikulskis A., Kuzdzal S., Golenko E., Petricoin E.F., 3rd, Liotta L.A., Patton W.F., Whiteley G.R., Rosenblatt K., Gurnani P., Nandi A., Neill S., Cullen S., O`Gorman M., Sarracino D., Lynch C., Johnson A., McKenzie W., Fishman D. (2007) Clin. Chem., 53, 1067-1074. Scholar google search
Whiteaker J.R., Zhang H., Eng J.K., Fang R., Piening B.D., Feng L.C., Lorentzen T.D., Schoenherr R.M., Keane J.F., Holzman T., Fitzgibbon M., Lin C., Cooke K., Liu T., Camp D.G., 2nd, Anderson L., Watts J., Smith R.D., McIntosh M.W., Paulovich A.G. (2007) J. Proteome Res., 6, 828-836. Scholar google search
Holland J.H. (1975) Adaptation in Natural and Artificial Systems. University of Michigan Press, Ann Arbor. Scholar google search
Hammer B., Strickert M., Villmann T. (2005) Neural Processing Letters, 21, 21-44. Scholar google search
Anderson N. L., Anderson N. G. (2002) Mol. Cell Proteomics, 1, 845-867. Scholar google search
Tammen H., Schulte I., Hess R., Menzel C., Kellmann M., Mohring T., Schulz-Knappe P. (2005) Proteomics, 5, 3414-3422. Scholar google search
Hsieh S.Y., Chen R.K., Pan Y.H., Lee H.L. (2006) Proteomics, 6, 3189-3198. Scholar google search
Baumann S., Ceglarek U., Fiedler G.M., Lembcke J., Leichtle A., Thiery J. (2005) Clin. Chem., 51, 973-980. Scholar google search
Geho D.H., Liotta L.A., Petricoin E.F., Zhao W., Araujo R.P. (2006) Curr. Opin. Chem. Biol., 10, 50-55. Scholar google search
Lopez M.F., Mikulskis A., Kuzdzal S., Bennett D.A., Kelly J., Golenko E., DiCesare J., Denoyer E., Patton W.F., Ediger R., Sapp L., Ziegert T., Lynch C., Kramer S., Whiteley G.R., Wall M.R., Mannion D.P., Della Cioppa G., Rakitan J.S., Wolfe G.M. (2005) Clin. Chem., 51, 1946-1954. Scholar google search
