The influence of adrenochrome and YC-1 on spermine NONO-induced activation of human soluble guanylyl cyclase was investigated. Adrenochrome (0.1-10 μM) had no effect on the basal activity, but it potentiated in concentration-dependent manner the spermine NONO-induced activation of this enzyme. Adrenochrome, like YC-1, sensitized guanylyl cyclase towards nitric oxide (NO) and produced the leftward shift of spermine NONO concentration responce curve. Addition of adrenochrome decreased the YC-1-induced leftward shift of spermine NONO concentration response curve. Adrenochrome also inhibited (by 63%) the enzyme activation by YC-1. These data demonstrates the possible competition between adrenochrome and YC-1. Thus, synergistic activation of NO-stimulated guanylyl cyclase activity by adrenochrome represents a new biochemical effect of this compound and indicates that adrenochrome may act as an endogenous regulator of NO-dependent stimulation of soluble guanylyl cyclase. This new property of adrenochrome, similar to YC-1, is necessary taking into account, especially under conditions of overproduction of adrenochrome in organism.
Severina I.S., Pyatakova N.V., Shchegolev A.Y., Sidorova T.A. (2008) YC-1-like potentiation of nitric oxide-dependent activation of soluble guanylyl cyclase by adrenochrome. Biomeditsinskaya Khimiya, 54(6), 679-686.
Severina I.S. et al. YC-1-like potentiation of nitric oxide-dependent activation of soluble guanylyl cyclase by adrenochrome // Biomeditsinskaya Khimiya. - 2008. - V. 54. -N 6. - P. 679-686.
Severina I.S. et al., "YC-1-like potentiation of nitric oxide-dependent activation of soluble guanylyl cyclase by adrenochrome." Biomeditsinskaya Khimiya 54.6 (2008): 679-686.
Severina, I. S., Pyatakova, N. V., Shchegolev, A. Y., Sidorova, T. A. (2008). YC-1-like potentiation of nitric oxide-dependent activation of soluble guanylyl cyclase by adrenochrome. Biomeditsinskaya Khimiya, 54(6), 679-686.
Kong L. Jiang Q.G., Qu Qs. (1989) Biomed. Environ Sci., 2, 72-77. Scholar google search
Genova M.L., Abd-Eisalam N.M., Mahdy el S.M., Bernacchia A., Lucarini M., Pedulli G.F., Lenaz G. (2006) Arch. Biochem. Biophys., 447, 167-173. Scholar google search
