Microbial spectrum and non-specific as well as specific IgA1 protease activity of isolated microorganisms were investigated in gingival liquid of patients with periodontitis.Microorganisms from the gingival liqud of these patients belonged to conditional-pathogenic obligate and facultatively anaerobic bacteria. 24 strains of microorganisms have been identified. Nonspecific proteolytic activity was found in the following microorganisms: Actinomyces israelii, Actinomyces naeslundii, Aerococcus viridans, Bifidobacterium longum, Neisseria subflave, Streptococcus parvulus, Eubacterium alactolyticum, Lactobaccilus catenoforme, Bacillus spp. Specific IgA1-protease activity and lack of proteolytic activity towards IgG was found in Streptococcus acidominimus, Streptococcus hansenii, Streptococcus salivarius, Leptotrychia buccalis, Staphylococcus haemolyticus and Neisseria sicca. No proteolytic activity was found in cultivation medium of Eubacterium alactolyticum (1 strain), Prevotella buccalis, Aerococcus viridans and Streptococcus sanguis.
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Keywords: periodontitis, a mouth, microflora, proteases, IgA1-protease, activity, ELISA
Citation:
Voropaeva E.A., Bajrakova A.L., Bichucher A.M., Kozlov L.V., D'yakov V.L. (2008) Proteolytic activity of microflora in oral cavity of patients with periodontitis. Biomeditsinskaya Khimiya, 54(6), 706-712.
Voropaeva E.A. et al. Proteolytic activity of microflora in oral cavity of patients with periodontitis // Biomeditsinskaya Khimiya. - 2008. - V. 54. -N 6. - P. 706-712.
Voropaeva E.A. et al., "Proteolytic activity of microflora in oral cavity of patients with periodontitis." Biomeditsinskaya Khimiya 54.6 (2008): 706-712.
Voropaeva, E. A., Bajrakova, A. L., Bichucher, A. M., Kozlov, L. V., D'yakov, V. L. (2008). Proteolytic activity of microflora in oral cavity of patients with periodontitis. Biomeditsinskaya Khimiya, 54(6), 706-712.
References
Теймуразов М.Г. (2006) Получение и некоторые свойства менингококковой IgA1-протеазы. Дисс. канд. наук, ФГУН Московский НИИ эпидемиологии и микробиологии им. Г.Н. Габричевского Роспотребнадзора, Москва. Scholar google search
Frandsen E.V.G., Kjeldsen M., Kilian M. (1997) Clin. Diagn. Lab. Immunol., 4, 458-464. Scholar google search
Kornfeld S. J., Plaut A.G. (1981) Rev. Infect. Dis., 3, 521-534. Scholar google search
Поздеев О.К. (2005) Медицинская микробиология, ГЭОТАР-Медиа, М. Scholar google search
Об унификации микробиологических (бактериологических) методов исследования, применяемых в клинико-диагностических лабораториях лечебно-профилактических учереждений (1989) Приказ МЗ СССР № 535 от 22 апреля 1985 года, М. Scholar google search
