Functional activity of glycolysis was studied in liver and skeletal muscles of rats subjected to acute alcohol intoxication. Alcohol administration in the dose of 1 g/kg led to inhibition of glycolytic enzymes activities and decreased levels of some glycolytic substrates in liver. Activity of LDH increased in skeletal muscle under these experimental conditions. Moderate dose of ethanol (2.5 g/kg) activated glucogenolysis and increased glucose level in liver. In this group inhibition of PFK activity and decrease of glucose-6-phosphate level in muscle was also observed. Alcohol administration in the dose of 5 g/kg caused inhibition of rate-limiting enzymes of glycolysis in liver and muscle.