1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences 2. Institute of Molecular Genetics RAS 3. Institut de biologie de Lille/Institut Pasteur
Immunogenicity for laboratory animals (rabbits and mice) of the whole hepatitis C virus envelope proteins and their conserved as well as hypervariable HVR1 sites has been investigated. Rabbit immune responses to HCV envelope proteins (both single E2 and E1E2 heterodimer) were shown to be much more efficient than murine immune responses. Upon the immunization of the rabbit with E2 protein, antibodies to several highly conserved linear B-epitopes of this protein as well as to the N-terminal fragment of the hypervariable region HVR1 were formed. Epitopes in the CR2 region were determined for the first time. Cross-reactivity was revealed between the N-terminal fragment of the protein E2 hypervariable region HVR1 and the octapeptide fragment of the protein E1 conserved region CR1, which shared four identical amino acid residues.
Download PDF:
Keywords: hepatitis C virus, envelope proteins, immune response, antibodies, B-epitopes
Citation:
Kuzmina T.I., Olenina L.V., Sanzhakov M.A., Farafonova T.E., Abramihina T.V., Dubuisson J., Sobolev B.N., Kolesanova E.F. (2009) Antigenicity and B-epitope mapping of hepatitis C virus envelope protein E2. Biomeditsinskaya Khimiya, 55(1), 32-40.
Kuzmina T.I. et al. Antigenicity and B-epitope mapping of hepatitis C virus envelope protein E2 // Biomeditsinskaya Khimiya. - 2009. - V. 55. -N 1. - P. 32-40.
Kuzmina T.I. et al., "Antigenicity and B-epitope mapping of hepatitis C virus envelope protein E2." Biomeditsinskaya Khimiya 55.1 (2009): 32-40.
Kuzmina, T. I., Olenina, L. V., Sanzhakov, M. A., Farafonova, T. E., Abramihina, T. V., Dubuisson, J., Sobolev, B. N., Kolesanova, E. F. (2009). Antigenicity and B-epitope mapping of hepatitis C virus envelope protein E2. Biomeditsinskaya Khimiya, 55(1), 32-40.
Bartosch B., Bukh J., Meunier J.-C., Granier C., Engle R.E., Blackwelder W.C., Emerson S.U., Cosset F.-L., Purcell R.H. (2003) Proc. Natl. Acad. Sci. USA, 100, 14199-14204. CrossRef Scholar google search
Keck Z.Y., Op de Beeck A., Hadlock K.G., Xia J., Li T.-K., Dubuisson J., Foung S.K.H. (2004) J. Virol., 78, 9224-9232. CrossRef Scholar google search
Ishii K., Rosa D., Watanabe Y., Katayama T., Harada H., Wyatt C., Kiyosawa K., Aizaki H., Matsuura Y., Houghton M., Abrignani S., Miyamura T. (1998) Hepatology, 28, 1117-1120. CrossRef Scholar google search
Yu M., Bartosch B., Zhang P., Guo P., Renzi P.M., Shen L., Granier C., Feinstone S.M., Cosset F.-L., Purcell R.H. (2004) Proc. Natl. Acad. Sci. USA, 101, 7705-7710. CrossRef Scholar google search
Lavillette D., Morice Y., Germanidis G., Donot P., Soulier A., Pagkalos E., Sakellariou G., Intrator L., Bartosch B., Pawlotsky J.-M., Cosset F.-L. (2005) J. Virol., 79, 6023-6034. CrossRef Scholar google search
Netski D.M., Mosbruger T., Depla E., Maertens G., Ray S.C., Hamilton R.G., Roundtree S., Thomas D.L., McKeating J., Cox A. (2005) Clin. Infect. Dis., 41, 667-675. CrossRef Scholar google search
Farci P., Shimoda A., Wong D., Cabezon T., De Gioannis D., Strazzera A., Shimizu Y., Shapiro M., Alter H.J., Purcell R.H. (1996) Proc. Natl. Acad. Sci. USA, 93, 15394-15399. CrossRef Scholar google search
Habersetzer F., Fournillier A., Dubuisson J., Rosa D., Abrignani S., Wychowski C., Nakano I., Trepo C., Desgranges C., Inchauspe G. (1998) Virology, 249, 32-41. CrossRef Scholar google search
Eren R., Landstein D., Terkieltaub D., Nussbaum O., Zauberman A. et al. (2006) J. Virol., 80, 2654-2664. Scholar google search
Rosa D., Campagnoli S., Moretto C., Guenzi E., Cousens L., Chin M., Dong C., Weiner A.J., Lau J.Y., Choo Q.L., Chien D., Pileri P., Houghton M., Abrignani S. (1996) Proc. Natl. Acad. Sci. USA, 93, 1759-1763. Scholar google search
Zibert A., Kraas W., Ross R.S., Meisel H., Lechner S., Jung G., Roggendorf M. (1999) J. Hepatol., 30, 177-184. Scholar google search
Carlos M.P., Yamamura Y., Vu Q., Conzen K., Anderson D.E., Torres J.V. (2004) Clin. Immunol., 111, 22-27. Scholar google search
Puntoriero G., Meola A., Lahm A., Zucchelli S., Ercole B.B., Tafi R., Pezzanera M., Mondelli M.U., Cortese R., Tramontano A., Galfre` G., Nicosia A. (1998) EMBO J., 17, 3521-3533. Scholar google search
Cocquerel L., Meunier J.-C., Pillez A., Wychowski C., Dubuisson J. (1998)J. Virol., 72, 2183-2191. Scholar google search
Cocquerel L., Op De Beeck A., Lambot M., Roussel J., Delgrange D., Pillez A., Wychowski C., Penin F., Dubuisson J. (2002) EMBO J., 21, 2893-2902. Scholar google search
