In the present paper we highlight the dependence of normalized mass spectrometry intensity on protein concentration. We have defined dynamic range of concentrations from 1 nM to 1mcM. It has been shown that the dependence holds linearity along the whole concentration range as for pure proteins as for proteins mixtures. Our observation revealed that the correlation curve possess protein-specific tangent between 1 nM and 1 mcM. The efficacy of the method evaluated by AQUA and 18O-labelling techniques of quantitative proteomics.
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Keywords: quantitative proteomics, mass-spectrometry, linear correlation, label-free method
Citation:
Kopylov A.T., Zgoda V.G., Archakov A.I. (2009) Label-free quantitative analysis of proteins using mass-spectrometry. Biomeditsinskaya Khimiya, 55(2), 125-139.
Kopylov A.T. et al. Label-free quantitative analysis of proteins using mass-spectrometry // Biomeditsinskaya Khimiya. - 2009. - V. 55. -N 2. - P. 125-139.
Kopylov A.T. et al., "Label-free quantitative analysis of proteins using mass-spectrometry." Biomeditsinskaya Khimiya 55.2 (2009): 125-139.
Kopylov, A. T., Zgoda, V. G., Archakov, A. I. (2009). Label-free quantitative analysis of proteins using mass-spectrometry. Biomeditsinskaya Khimiya, 55(2), 125-139.
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