Homogeneous preparations of liver glutathione reductase (GR, EC 1.6.4.2.), obtained from control rats and animals with toxic hepatitis, catalytic properties of this enzyme have been investigated. A number of the enzyme properties did not change under conditions of hepatitis These included electrophoretic mobility (Rf=0,23±0,01), molecular mass (104±5,20 kDa), pH-optimum (7,4±0,37). There was similarity in рК values of functional groups. At the same time, decrease in affinity of enzyme to a substrate and coenzyme and occurrence substrate-linked inhibition was observed under conditions of this pathology. There were some differencies in regulation of GR activity by metabolites of tricarboxylic acid cycle.
Agarkov A.A., Popova T.N., Semenikhina A.V. (2009) Catalytic properties of glutathione reductase from rats liver at norm and toxic hepatites. Biomeditsinskaya Khimiya, 55(2), 169-176.
Agarkov A.A. et al. Catalytic properties of glutathione reductase from rats liver at norm and toxic hepatites // Biomeditsinskaya Khimiya. - 2009. - V. 55. -N 2. - P. 169-176.
Agarkov A.A. et al., "Catalytic properties of glutathione reductase from rats liver at norm and toxic hepatites." Biomeditsinskaya Khimiya 55.2 (2009): 169-176.
Agarkov, A. A., Popova, T. N., Semenikhina, A. V. (2009). Catalytic properties of glutathione reductase from rats liver at norm and toxic hepatites. Biomeditsinskaya Khimiya, 55(2), 169-176.
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