Potentiation of nitric oxide-dependеnt activation of soluble guanylate cyclase by laevomycetin, tetracycline and oxolin

   
Shchegolev A.Yu.1, Sidorova T.A.2, Severina I.S.1

1. Orechovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
2. Blokhin Cancer Center, Russian Academy of Medical Sciences
Section: Experimental/Clinical Study
UDK: 613.632.615.36      PubMed Id: 19663005
Year: 2009  Volume: 55  Issue: 3  Pages: 331-337
The influence of antibiotics laevomycetin and tetracycline and the antivirus agent oxolin on the activity of human platelet soluble guanylate cyclase, the stimulation of the enzyme by NO-donors (sodium nitroprusside (SNP) and spermine nanoate (spermine NONO)) and the combination of spermine NONO and YC-1 was investigated. All preparations used in the concentration range 0,1-10 мM had no effect on the basal activity of guanylate cyclase but potentiated the SNP-induced activation of this enzyme. All preparations used synergistically increased (similar to YC-1) spermine NONO-induced activation of soluble guanylate cyclase. At the same time these compounds did not produce the leftward shift of spermine NONO concentration response curve characteristic for YC-1. Moreover, all compounds used did not influence the leftward shift of spermine NONO concentration response curve obtained in the presence of YC-1. This demonstrated that there was no competition between YC-1 and the drugs for interaction with the enzyme. The revealed regulatory phenomen of laevomycetin, tetracycline and oxolin to increase synergistically NO-dependent activation of soluble guanylate cyclase may cause additional pharmacological effects during prolong treatment by these drugs. This fact is necessary taking into account.
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Shchegolev A.Yu., Sidorova T.A., Severina I.S. (2009) Biomeditsinskaya khimiya, 55(3), 331-337.
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