A new method for quantitative estimation of the number of virus particles

Shebanova A.S.; Savvateev M.N.; Maluchenko N.V.; Trofimov D.Yu.; Agapov I.I.

A new method for quantitative estimation of the number of virus particles

Shebanova A.S.¹, Savvateev M.N.², Maluchenko N.V.¹, Trofimov D.Yu.³, Agapov I.I.⁴

1. Lomonosov Moscow State University, Biological Department
2. NT-MDT Co., State Research Institute of Physical Problems
3. DNA-Technology Co
4. Lomonosov Moscow State University, Biological Department Research Institute of Transplantology and Artificial Organs

Section: Experimental/Clinical Study

PubMed Id: 20017392

Year: 2009 Volume: 55 Issue: 5 Pages: 610-620

In the present work virus particles of live mumps virus vaccine widely used for vaccination in Russia have been detected and visualized by atomic force microscopy. For quantitative estimation of the number of observed virus particles the special method has been proposed. The presence of protein component of the virus in vaccine was tested by ELISA and dot-blot analysis. Using quantitative real-time PCR assay the number of copies of viral RNA was estimated. The results of quantitative estimation obtained by real-time PCR corresponded with atomic force microscopy data.

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Keywords: atomic force microscopy, live virus vaccine, virus particles, quantitative real-time PCR

Citation:

Shebanova, A. S., Savvateev, M. N., Maluchenko, N. V., Trofimov, D. Yu., Agapov, I. I. (2009). A new method for quantitative estimation of the number of virus particles. Biomeditsinskaya Khimiya, 55(5), 610-620.

This paper is also available as the English translation: 10.1134/S199075080903010X

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