1. Institute of Biomedical Chemistry RAMS 2. Scientific Association for High Temperatures RAS 3. JSV "VECTOR-BEST" 4. Russian Research Center of Molecular Diagnostics & Therapy
The aim of the study was to demonstrate the possibility of detection of serological markers, containing the hepatitis B surface antigen (HBsAg) and hepatitis C virus core-antigen (HCVcoreAg) in human serum, by use of a new atomic force microscopy (AFM)-based nanotechnological approach.In this study, the immobilization on AFM-chip of antibodies against the hepatitis B virus surface antigen (anti-HBsAg) as well as the antibodies against the hepatitis C virus core antigen (anti-HCVcoreAg) was performed. It was shown that such approach enables to detect HBsAg, HCVcoreAg and the viral fragments containing these antigens in the serum. Comparative analysis of detection of HBsAg- and HCVcoreAg - containing particles by use of the AFM method vs. traditional methods (ELISA, PCR) has demonstrated the 75% coincidence of results between the AFM and the latter two methods.
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Keywords: atomic-force microscopy, hepatitis B virus surface antigen (HBsAg), hepatitis C virus core antigen (HCVcoreAg)
Citation:
Ivanov Yu.D., Frantsuzov P.A., Pleshakova T.O., Ziborov V.S., Svetlov S.K., Krohin N.V., Konev V.A., Kovalev O.B., Uchaikin V.F., Yastrebova O.N., Sveshnikov P.G., Archakov A.I. (2009) Atomic force microscopy detection of serological markers of viral hepatites B and C. Biomeditsinskaya Khimiya, 55(6), 689-701.
Ivanov Yu.D. et al. Atomic force microscopy detection of serological markers of viral hepatites B and C // Biomeditsinskaya Khimiya. - 2009. - V. 55. -N 6. - P. 689-701.
Ivanov Yu.D. et al., "Atomic force microscopy detection of serological markers of viral hepatites B and C." Biomeditsinskaya Khimiya 55.6 (2009): 689-701.
Ivanov, Yu. D., Frantsuzov, P. A., Pleshakova, T. O., Ziborov, V. S., Svetlov, S. K., Krohin, N. V., Konev, V. A., Kovalev, O. B., Uchaikin, V. F., Yastrebova, O. N., Sveshnikov, P. G., Archakov, A. I. (2009). Atomic force microscopy detection of serological markers of viral hepatites B and C. Biomeditsinskaya Khimiya, 55(6), 689-701.
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