The aim of the study was to demonstrate the possibility of detection of serological markers, containing the hepatitis B surface antigen (HBsAg) and hepatitis C virus core-antigen (HCVcoreAg) in human serum, by use of a new atomic force microscopy (AFM)-based nanotechnological approach.In this study, the immobilization on AFM-chip of antibodies against the hepatitis B virus surface antigen (anti-HBsAg) as well as the antibodies against the hepatitis C virus core antigen (anti-HCVcoreAg) was performed. It was shown that such approach enables to detect HBsAg, HCVcoreAg and the viral fragments containing these antigens in the serum. Comparative analysis of detection of HBsAg- and HCVcoreAg - containing particles by use of the AFM method vs. traditional methods (ELISA, PCR) has demonstrated the 75% coincidence of results between the AFM and the latter two methods.
Keywords: atomic-force microscopy, hepatitis B virus surface antigen (HBsAg), hepatitis C virus core antigen (HCVcoreAg)
Ivanov Yu.D. et al. Atomic force microscopy detection of serological markers of viral hepatites B and C // Biomeditsinskaya khimiya. - 2009. - V. 55. -N 6. - P. 689-701.
Ivanov Yu.D. et al., "Atomic force microscopy detection of serological markers of viral hepatites B and C." Biomeditsinskaya khimiya 55.6 (2009): 689-701.
Ivanov, Yu. D., Frantsuzov, P. A., Pleshakova, T. O., Ziborov, V. S., Svetlov, S. K., Krohin, N. V., Konev, V. A., Kovalev, O. B., Uchaikin, V. F., Yastrebova, O. N., Sveshnikov, P. G., Archakov, A. I. (2009). Atomic force microscopy detection of serological markers of viral hepatites B and C. Biomeditsinskaya khimiya, 55(6), 689-701.