Atomic force microscopy detection of serological markers of viral hepatites B and C

Ivanov Yu.D.1 , Frantsuzov P.A.1, Pleshakova T.O.1, Ziborov V.S.2, Svetlov S.K.1, Krohin N.V.1, Konev V.A.1, Kovalev O.B.1, Uchaikin V.F.1, Yastrebova O.N.3, Sveshnikov P.G.4, Archakov A.I.1

1. Institute of Biomedical Chemistry RAMS
2. Scientific Association for High Temperatures RAS
4. Russian Research Center of Molecular Diagnostics & Therapy
Section: Experimental/Clinical Study
PubMed Id: 20469717
Year: 2009  Volume: 55  Issue: 6  Pages: 689-701
The aim of the study was to demonstrate the possibility of detection of serological markers, containing the hepatitis B surface antigen (HBsAg) and hepatitis C virus core-antigen (HCVcoreAg) in human serum, by use of a new atomic force microscopy (AFM)-based nanotechnological approach.In this study, the immobilization on AFM-chip of antibodies against the hepatitis B virus surface antigen (anti-HBsAg) as well as the antibodies against the hepatitis C virus core antigen (anti-HCVcoreAg) was performed. It was shown that such approach enables to detect HBsAg, HCVcoreAg and the viral fragments containing these antigens in the serum. Comparative analysis of detection of HBsAg- and HCVcoreAg - containing particles by use of the AFM method vs. traditional methods (ELISA, PCR) has demonstrated the 75% coincidence of results between the AFM and the latter two methods.
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Keywords: atomic-force microscopy, hepatitis B virus surface antigen (HBsAg), hepatitis C virus core antigen (HCVcoreAg)

Ivanov, Yu. D., Frantsuzov, P. A., Pleshakova, T. O., Ziborov, V. S., Svetlov, S. K., Krohin, N. V., Konev, V. A., Kovalev, O. B., Uchaikin, V. F., Yastrebova, O. N., Sveshnikov, P. G., Archakov, A. I. (2009). Atomic force microscopy detection of serological markers of viral hepatites B and C. Biomeditsinskaya Khimiya, 55(6), 689-701.
This paper is also available as the English translation: 10.1134/S1990750810020010
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