Application of the method of vital in vivo fluorescence intensity measurement in biological tissues for pharmacokinetic studies of different chlorin-based photosensitisers
A precise and reproducible method of quantitative determination of the photosensitizer Photolon® in hepatic tissue of the liver of rats by means of spectrophotometric assay with preliminary chemical extraction was developed for validation of the method of in vivo measurement of a fluorescent signal of the tissue after injection of the photosensitizer. Using the ex vivo spectrophotometric technique a quantitative assessment of the results of spectrofluorescent measurements performed in vivo was made. A high correlation (R2=0,99) between results estimated by two different techniques was shown. Also validity of the simple and easy-to-use method of in vivo fluorescence intensity measurements for the investigation of the pharmacokinetics and biodistribution of different photosensitizers was demonstrated.
Shliakhtin S.V., Trukhachova T.V., Isakau G.A., Istomin Y.P. (2009) Application of the method of vital in vivo fluorescence intensity measurement in biological tissues for pharmacokinetic studies of different chlorin-based photosensitisers. Biomeditsinskaya Khimiya, 55(6), 766-778.
Shliakhtin S.V. et al. Application of the method of vital in vivo fluorescence intensity measurement in biological tissues for pharmacokinetic studies of different chlorin-based photosensitisers // Biomeditsinskaya Khimiya. - 2009. - V. 55. -N 6. - P. 766-778.
Shliakhtin S.V. et al., "Application of the method of vital in vivo fluorescence intensity measurement in biological tissues for pharmacokinetic studies of different chlorin-based photosensitisers." Biomeditsinskaya Khimiya 55.6 (2009): 766-778.
Shliakhtin, S. V., Trukhachova, T. V., Isakau, G. A., Istomin, Y. P. (2009). Application of the method of vital in vivo fluorescence intensity measurement in biological tissues for pharmacokinetic studies of different chlorin-based photosensitisers. Biomeditsinskaya Khimiya, 55(6), 766-778.
Glanzmann T., Hadjur C., Zellweger M., Grosiean P., Forrer M., Ballini J., Monnier P., van den Bergh H., Lim C., Wagnieres G. (1998) Photochem. Photobiol., 67, pp. 596-602. Scholar google search
Panjehpour M., Sneed R.E., Frazier D.L., Barnhill M.A., O`Brien S.F., Harb W., Overholt B.F. (1993) J. Lasers Surg. Med., 13, 23-30. CrossRef Scholar google search
Stepp H., Beck T., Beyer W., Pfaller C., Schuppler M., Sroka R., Baumgartner R. (2007) Med. Laser Appl., 22, 23-34. CrossRef Scholar google search
Isakau H.A., Trukhacheva T.V., Zhebentyaev A.I., Petrov P.T. (2007) J. Biomed. Chromatogr., 21, 318-325. CrossRef Scholar google search
Moan J., Ma L.W., Juzeniene A., Iani V., Juzenas P., Apricena F., Teng Q. (2003) Int. J. Cancer, 103, 132-135. CrossRef Scholar google search
Wang Z.J., He Y.Y., Huang C.G., Huang J.S., Huang Y.C., An J.Y. et al. (1999) Photochem. Photobiol., 70, 773-780. CrossRef Scholar google search
Shah V.P. (2001) Guidance for Industry: Bioanalytical Methods Validation. US Department of Health and Human Services, Food and Drug Administration, CDER, Rockville, USA, May 2001. Scholar google search
Mang T., Kost J., Sullivan M., Wilson B.C. (2006) J. Photodiagnosis and Photodynamic Therapy, 3, 168-176. CrossRef Scholar google search
