Application of the method of vital in vivo fluorescence intensity measurement in biological tissues for pharmacokinetic studies of different chlorin-based photosensitisers

   
Shliakhtin S.V.1 , Trukhachova T.V.1, Isakau G.A.1, Istomin Y.P.2

1. RUE "Belmedpreparaty"
2. National Center Of Oncology
Section: Experimental/Clinical Study
UDK: 57.085.1      PubMed Id: 20469725
Year: 2009  Volume: 55  Issue: 6  Pages: 766-778
A precise and reproducible method of quantitative determination of the photosensitizer Photolon® in hepatic tissue of the liver of rats by means of spectrophotometric assay with preliminary chemical extraction was developed for validation of the method of in vivo measurement of a fluorescent signal of the tissue after injection of the photosensitizer. Using the ex vivo spectrophotometric technique a quantitative assessment of the results of spectrofluorescent measurements performed in vivo was made. A high correlation (R2=0,99) between results estimated by two different techniques was shown. Also validity of the simple and easy-to-use method of in vivo fluorescence intensity measurements for the investigation of the pharmacokinetics and biodistribution of different photosensitizers was demonstrated.
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Shliakhtin S.V., Trukhachova T.V., Isakau G.A., Istomin Y.P. (2009) Biomeditsinskaya khimiya, 55(6), 766-778.
This paper is also available as the English translation:10.1134/S1990750810020095
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