Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.
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Keywords: atomic force microscopy, mass spectrometry, HCVcoreAg
Citation:
Kaysheva A.L., Ivanov Yu.D., Zgoda V.G., Frantsuzov P.A., Pleshakova T.O., Krohin N.V., Ziborov V.S., Archakov A.I. (2010) Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis. Biomeditsinskaya Khimiya, 56(1), 26-39.
Kaysheva A.L. et al. Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis // Biomeditsinskaya Khimiya. - 2010. - V. 56. -N 1. - P. 26-39.
Kaysheva A.L. et al., "Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis." Biomeditsinskaya Khimiya 56.1 (2010): 26-39.
Kaysheva, A. L., Ivanov, Yu. D., Zgoda, V. G., Frantsuzov, P. A., Pleshakova, T. O., Krohin, N. V., Ziborov, V. S., Archakov, A. I. (2010). Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis. Biomeditsinskaya Khimiya, 56(1), 26-39.
