Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis

Kaysheva A.L.1 , Ivanov Yu.D.1, Zgoda V.G.1, Frantsuzov P.A.1, Pleshakova T.O.1, Krohin N.V.1, Ziborov V.S.2, Archakov A.I.1

1. Institute of Biomedical Chemistry RAMS
2. Joint Institute for High Temperatures RAS
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20105601026      UDK: 577.152.3      PubMed Id: 21328909
Year: 2010  Volume: 56  Issue: 1  Pages: 26-39
Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.
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Kaysheva A.L., Ivanov Yu.D., Zgoda V.G., Frantsuzov P.A., Pleshakova T.O., Krohin N.V., Ziborov V.S., Archakov A.I. (2010) Biomeditsinskaya khimiya, 56(1), 26-39.
This paper is also available as the English translation:10.1134/S1990750810010038
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