The ability of artificial ribonucleases to cause in the concentration-dependent manner death of cancer cells has been studied. The cytotoxic activity of artificial ribonucleases is observed at rather low concentration of these compounds (10-5 М). Analysis of the mechanism of artificial ribonucleases citotoxicity revealed that compounds under the study exhibit membranotropic activity in addition to ribonucleases activity found earlier. This activity is responsible for effective penetration of these compounds inside cells. The results obtained show that artificial ribonucleases induce cell death via damage of cells membrane, detachment of plasmalemma and derangement its macromolecular organization. In the case of short-term exposure of cells to the compounds, cells, even with damaged membrane, survive.
Logashenko E.B., Kuznetsova I.L., Ryabchikova E.I., Vlassov V.V., Zenkova M.A. (2010) Mechanism of the toxicity of the artificial ribonucleases for the different human cancer cell lines. Biomeditsinskaya Khimiya, 56(2), 230-243.
Logashenko E.B. et al. Mechanism of the toxicity of the artificial ribonucleases for the different human cancer cell lines // Biomeditsinskaya Khimiya. - 2010. - V. 56. -N 2. - P. 230-243.
Logashenko E.B. et al., "Mechanism of the toxicity of the artificial ribonucleases for the different human cancer cell lines." Biomeditsinskaya Khimiya 56.2 (2010): 230-243.
Logashenko, E. B., Kuznetsova, I. L., Ryabchikova, E. I., Vlassov, V. V., Zenkova, M. A. (2010). Mechanism of the toxicity of the artificial ribonucleases for the different human cancer cell lines. Biomeditsinskaya Khimiya, 56(2), 230-243.
Zenkova M., Beloglazova N., Sil`nikov V., Vlassov V., Giege R. (2001) Methods Enzymol., 341, 468-490. CrossRef Scholar google search
Koval`ov N., Kuznetsova I., Burakova E., Sil`nikov V., Zenkova M., Vlassov V. (2004) Nucleosides Nucleotides Nucleic Acids, 23, 977-981. CrossRef Scholar google search
Chattopadhyay N., Kher R., Godbole M. (1993) Biotechniques, 15, 24-26. Scholar google search
Kostenko E.V., Laktionov P.P., Vlassov V.V., Zenkova M.A. (2002) Biochim. Biophys. Acta, 1576, 143-147. CrossRef Scholar google search
Park J.G., Kramer B.S., Steinberg S.M., Carmichael J., Collins J.M., Minna J.D., Gazdar A.F. (1987) Cancer Res., 47, 5875-5879. Scholar google search
Uikli B. (1975) Elektronnaya mikroskopiya dlya nachinayushhikh, Mir, Moskva. Scholar google search
Spalletti-Cernia D., Sorrentino R., Di Gaetano S., Piccoli R., Santoro M., D`Alessio G., Laccetti P., Vecchio G. (2004) Br. J. Cancer, 90, 270-277. CrossRef Scholar google search
Lee I., Kalota A., Gewirtz A.M., Shogen K. (2007) Anticancer Res., 27(1A), 299-307. Scholar google search
