1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences 2. Russian Scientific Center of Roentgen-Radiology, Ministry of Health and Social Development
Quantitative and qualitative changes in circulating extracellular DNA (ec-DNA) of blood plasma are considered as markers for diagnosis and prognostic of tumor pathology. We investigated the content of mutant copies of the circulating extracellular mitochondrial DNA (ec-mtDNA) in blood plasma (using the enzymatic method, based on the cleavage of DNA with unpaired bases by CEL-I endonuclease) in 8 patients with lung cancer before and after radiotherapy, as well as in healthy young and elderly donors. It was found that in the plasma of healthy elderly donors share of ec-mtDNA with mutations (consisting of total circulating DNA) is much greater, than that of young donors. On the other hand, in the plasma of lung cancer patients (aged 70-76 years) before radiotherapy a substantial increase in the share of ec-mtDNA with mutations, compared with that of healthy elderly donors. Following radiotherapy, patients with lung cancer found a twofold increase of the proportion of ec-mtDNA with mutations in the total circulating plasma DNA. This increase is largely, perhaps due to the release of ec-mtDNA with mutations from dying tumor cells and cells damaged by normal tissues.
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Keywords: lung cancer, extracellular mitochondrial DNA in blood plasma, radiotherapy, mutations, CEL-I endonuclease, splitting of heteroduplexes
Citation:
Strelkova I.Y., Abdullaev S.A., Snigireva G.P., Bezlepkin V.G., Gaziev A.I. (2010) Share of extracellular mitochondrial DNA with a mutations increases in the plasma of patients lung cancer after radiotherapy. Biomeditsinskaya Khimiya, 56(4), 517-525.
Strelkova I.Y. et al. Share of extracellular mitochondrial DNA with a mutations increases in the plasma of patients lung cancer after radiotherapy // Biomeditsinskaya Khimiya. - 2010. - V. 56. -N 4. - P. 517-525.
Strelkova I.Y. et al., "Share of extracellular mitochondrial DNA with a mutations increases in the plasma of patients lung cancer after radiotherapy." Biomeditsinskaya Khimiya 56.4 (2010): 517-525.
Strelkova, I. Y., Abdullaev, S. A., Snigireva, G. P., Bezlepkin, V. G., Gaziev, A. I. (2010). Share of extracellular mitochondrial DNA with a mutations increases in the plasma of patients lung cancer after radiotherapy. Biomeditsinskaya Khimiya, 56(4), 517-525.
Chiu R.W., Chan L.Y., Lam N.Y., Tsui N.B., Ng E.K., Rainer T.H., Lo Y.M. (2003) Clin. Chem., 49, 719-726. CrossRef Scholar google search
Stemmer C., Beau-Faller M., Pencreach E., Guerin E., Schneider A., Jaqmin D., Quoix E., Gaub M., Oudet P. (2003) Clin. Chem., 49, 1953-1955. CrossRef Scholar google search
Pagniez-Mammeri H., Lombes A., Brivet M., Ogier-de Baulny H., Landrieu P., Legrand A., Slama A. (2009) Mol. Genet. Metab., 96, 196-200. CrossRef Scholar google search
Paci M., Maramotti S., Bellesia E., Formisano D., Albertazzi L., Ricchetti T., Ferrari G., Annessi V., Lasagni D., Carbonelli C., De Franco S., Brini M., Sgarbi G., Lodi R. (2009) Lung Cancer., 64, 92-97. CrossRef Scholar google search
