Microencapsulated multicellular tumor spheroids:preparation and use as a novel in vitro model for drug screening

Tsoy A.M.1 , Zaytseva-Zotova D.S.1, Edelweiss E.F.1, Bartkowiak A.2, Goergen J-L.3, Vodovozova E.L.1, Markvicheva E.A.1

1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
2. Westpomeranian University of Technology
3. Institut National Polytechnique de Lorraine - ENSAIA
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20105606674      PubMed Id: 21395070
Year: 2010  Volume: 56  Issue: 6  Pages: 674-685
In the current study a technique for microencapsulation of human breast adenocarcinoma cells MCF-7 in alginate-chitosan microcapsules is used. Microencapsulation is proposed to generate multicellular tumor spheroids (MTS) based on these cells and to test them further as an in vitro model for anti-tumor drug screening. Cytotoxicity of methotrexate (MTX) was studied on the obtained MTS. A set of MTS with mean size of 150, 200 and 300 m was prepared in function of a cultivation time. After incubation of MTS in cultivation medium containing MTX at concentrations of 1, 2, 10, 50 and 100 nM for 48 hs cell viability was evaluated. MTS were shown to be more resistant to MTX than the monolayer culture, and the resistance to MTX was increased with enhancing a spheroid size. At MTX concentration of 100 nM a number of viable cells in MTS with the size of 300 m was 2.5-fold bigger than that one in monolayer culture. It is suggested that the cells in microencapsulated MTS can better mimic cell behavior in a small size solid tumor than the cells in a monolayer culture. In future microencapsulated MTS can be proposed as a novel in vitro model for anticancer drug screening.
Download PDF:  
Keywords: microencapsulation, multicellular tumor spheroids, alginate-chitosan microcapsules, methotrexate

Tsoy, A. M., Zaytseva-Zotova, D. S., Edelweiss, E. F., Bartkowiak, A., Goergen, J-L., Vodovozova, E. L., Markvicheva, E. A. (2010). Microencapsulated multicellular tumor spheroids:preparation and use as a novel in vitro model for drug screening. Biomeditsinskaya Khimiya, 56(6), 674-685.
This paper is also available as the English translation: 10.1134/S1990750810030054
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)