Microencapsulated multicellular tumor spheroids:preparation and use as a novel in vitro model for drug screening

Tsoy A.M.1 , Zaytseva-Zotova D.S.1, Edelweiss E.F.1, Bartkowiak A.2, Goergen J-L.3, Vodovozova E.L.1, Markvicheva E.A.1

1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
2. Westpomeranian University of Technology
3. Institut National Polytechnique de Lorraine - ENSAIA
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20105606674      PubMed Id: 21395070
Year: 2010  Volume: 56  Issue: 6  Pages: 674-685
In the current study a technique for microencapsulation of human breast adenocarcinoma cells MCF-7 in alginate-chitosan microcapsules is used. Microencapsulation is proposed to generate multicellular tumor spheroids (MTS) based on these cells and to test them further as an in vitro model for anti-tumor drug screening. Cytotoxicity of methotrexate (MTX) was studied on the obtained MTS. A set of MTS with mean size of 150, 200 and 300 m was prepared in function of a cultivation time. After incubation of MTS in cultivation medium containing MTX at concentrations of 1, 2, 10, 50 and 100 nM for 48 hs cell viability was evaluated. MTS were shown to be more resistant to MTX than the monolayer culture, and the resistance to MTX was increased with enhancing a spheroid size. At MTX concentration of 100 nM a number of viable cells in MTS with the size of 300 m was 2.5-fold bigger than that one in monolayer culture. It is suggested that the cells in microencapsulated MTS can better mimic cell behavior in a small size solid tumor than the cells in a monolayer culture. In future microencapsulated MTS can be proposed as a novel in vitro model for anticancer drug screening.
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Keywords: microencapsulation, multicellular tumor spheroids, alginate-chitosan microcapsules, methotrexate

Tsoy, A. M., Zaytseva-Zotova, D. S., Edelweiss, E. F., Bartkowiak, A., Goergen, J-L., Vodovozova, E. L., Markvicheva, E. A. (2010). Microencapsulated multicellular tumor spheroids:preparation and use as a novel in vitro model for drug screening. Biomeditsinskaya khimiya, 56(6), 674-685.
This paper is also available as the English translation: 10.1134/S1990750810030054
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