A modified RP-HPLC-MS approach has been proposed for a single run separation and identification of the molecular species of different phospholipid classes in a complex extract. This approach has been applied to the analysis of glycero- and sphingolipid composition of human erythrocytes and a number of ceramide fractions have been identified; these fractions was missed in previous studies employing similar methods. The fine experimental design leads to the decrease in the number of procedures needed for a complete phospholipid profiling of the sample.
Korzhenevskiy D.A., Selischeva A.A., Saveliev S.V. (2010) An approach to the identification of the phospholipid molecular species in human erythrocytes using HPLC with mass-spectrometric detection. Biomeditsinskaya Khimiya, 56(6), 747-757.
Korzhenevskiy D.A. et al. An approach to the identification of the phospholipid molecular species in human erythrocytes using HPLC with mass-spectrometric detection // Biomeditsinskaya Khimiya. - 2010. - V. 56. -N 6. - P. 747-757.
Korzhenevskiy D.A. et al., "An approach to the identification of the phospholipid molecular species in human erythrocytes using HPLC with mass-spectrometric detection." Biomeditsinskaya Khimiya 56.6 (2010): 747-757.
Korzhenevskiy, D. A., Selischeva, A. A., Saveliev, S. V. (2010). An approach to the identification of the phospholipid molecular species in human erythrocytes using HPLC with mass-spectrometric detection. Biomeditsinskaya Khimiya, 56(6), 747-757.
