Doxorubicin-resistant MCF-7Dox line, which is a derivative of the drug-sensitive MCF-7 human breast carcinima line, differs from the latter by a strongly reduced expression of the α2β1 integrin and a highly increased expression of the α5β1 receptor. Silencing of this integrin in the MCF-7Dox cells by transfection with α5-specific siRNA markedly stimulated anoikis and increased sensitivity of the cells to doxorubicin. α5β1 silencing also leads to significant inhibition of the activity of kinases Akt and Erk2 in MCF-7Dox cells. Our results suggest that integrins α5β1-induced signals, controlling distinct aspects of cell behavior, are conducted through the common signal pathways.
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Keywords: integrins, anoikis, drug resistance, signal protein kinases
Citation:
Morozevich G.E., Kozlova N.I., Ushakova N.A., Preobrazhenskaya M.E., Berman A.E. (2011) Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance. Biomeditsinskaya Khimiya, 57(1), 77-84.
Morozevich G.E. et al. Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance // Biomeditsinskaya Khimiya. - 2011. - V. 57. -N 1. - P. 77-84.
Morozevich G.E. et al., "Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance." Biomeditsinskaya Khimiya 57.1 (2011): 77-84.
Morozevich, G. E., Kozlova, N. I., Ushakova, N. A., Preobrazhenskaya, M. E., Berman, A. E. (2011). Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance. Biomeditsinskaya Khimiya, 57(1), 77-84.
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