1. M.V. Lomonosov Moscow State University, Chemistry Faculty, Department of Chemical Enzymology
ZAO "NVO Immunotek" 2. National Research Centre of Surgery RAMS, Laboratory of Immunology and Regulatory Mechanisms 3. M.V. Lomonosov Moscow State University, Chemistry Faculty, Department of Chemical Enzymology
A test-system based on enzyme-linked immunosorbent assay (ELISA) for quantitative determination of cyclosporin A (CSA) in human whole blood has been developed. The detection limit of the method was 25 ng/ml, the linearity of the method in the concentration range 60-1400 ng/ml varied from 94 to 105%, the variation coefficient did not exceed 8%. The novel method exhibited good correlation with radio-immunoassay and polarization fluoroimmunoassay methods; the linear regression coefficients were 0.965 and 0.983, respectively. The developed test system is stable for at least 9 months when stored at 4°C and can be used in clinical practice.
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Keywords: cyclosporin A, enzyme-linked immunosorbent assay (ELISA)
Citation:
Andreeva I.P., Vorobyeva N.T., Vinnitsky L.I., Bogush S.S., Gavrilova E.M., Egorov A.M. (2011) Enzyme-linked immunosorbent assay for determination of cyclosporin a in whole blood. Biomeditsinskaya Khimiya, 57(5), 554-561.
Andreeva I.P. et al. Enzyme-linked immunosorbent assay for determination of cyclosporin a in whole blood // Biomeditsinskaya Khimiya. - 2011. - V. 57. -N 5. - P. 554-561.
Andreeva I.P. et al., "Enzyme-linked immunosorbent assay for determination of cyclosporin a in whole blood." Biomeditsinskaya Khimiya 57.5 (2011): 554-561.
Andreeva, I. P., Vorobyeva, N. T., Vinnitsky, L. I., Bogush, S. S., Gavrilova, E. M., Egorov, A. M. (2011). Enzyme-linked immunosorbent assay for determination of cyclosporin a in whole blood. Biomeditsinskaya Khimiya, 57(5), 554-561.
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